ArticleNucleic acids research2006
The uORF-containing thrombopoietin mRNA escapes nonsense-mediated decay (NMD).
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- An upstream open reading frame represses translation of the neuronal potassium channel KCNQ2.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Genomic stop codon scanning reveals quantitative principles of nonsense-mediated mRNA decay.bioRxiv : the preprint server for biology · 2025Article
- Nonsense-mediated mRNA decay: a key regulatory system engaged in cancer.Cell communication and signaling : CCS · 2025Review
- Ribosome stalling-induced NIP5;1 mRNA decay triggers ARGONAUTE1-dependent transcription downregulation.Nucleic acids research · 2025Article
- Article
- Transcript-specific characteristics determine the contribution of endo- and exonucleolytic decay pathways during the degradation of nonsense-mediated decay substrates.RNA (New York, N.Y.) · 2017Article
- The role of nucleotide composition in premature termination codon recognition.BMC bioinformatics · 2016Article
- Aberrant splicing induced by the most common EPG5 mutation in an individual with Vici syndrome.Brain : a journal of neurology · 2016Article
- Physiological and pathophysiological role of nonsense-mediated mRNA decay.Pflugers Archiv : European journal of physiology · 2016Review
- Mechanism, factors, and physiological role of nonsense-mediated mRNA decay.Cellular and molecular life sciences : CMLS · 2015Review
- Article
- Identification of novel post-transcriptional features in olfactory receptor family mRNAs.Nucleic acids research · 2015Article
- Regulation of natural mRNAs by the nonsense-mediated mRNA decay pathway.Eukaryotic cell · 2014Review
- Nonsense-mediated mRNA decay immunity can help identify human polycistronic transcripts.PloS one · 2014Article
- Global analyses of UPF1 binding and function reveal expanded scope of nonsense-mediated mRNA decay.Genome research · 2013Article
- A perspective on mammalian upstream open reading frame function.The international journal of biochemistry & cell biology · 2013Review
- Intron Retention and TE Exonization Events in ZRANB2.Comparative and functional genomics · 2012Article
- Mechanism of escape from nonsense-mediated mRNA decay of human beta-globin transcripts with nonsense mutations in the first exon.RNA (New York, N.Y.) · 2011Article
- Article
- Expression proteomics of UPF1 knockdown in HeLa cells reveals autoregulation of hnRNP A2/B1 mediated by alternative splicing resulting in nonsense-mediated mRNA decay.BMC genomics · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Platelet production is induced by the cytokine thrombopoietin (TPO). It is physiologically critical that TPO expression is tightly regulated, because lack of TPO causes life-threatening thrombocytopenia while an excess of TPO results in thrombocytosis. The plasma concentration of TPO is controlled by a negative feedback loop involving receptor-mediated uptake of TPO by platelets. Furthermore, TPO biosynthesis is limited by upstream open reading frames (uORFs) that curtail the translation of the TPO mRNA. uORFs are suggested to activate RNA degradation by nonsense-mediated decay (NMD) in a number of physiological transcripts. Here, we determine whether NMD affects TPO expression. We show that reporter mRNAs bearing the seventh TPO uORF escape NMD. Importantly, endogenously expressed TPO mRNA from HuH7 cells is unaffected by abrogation of NMD by RNAi. Thus, regulation of TPO expression is independent of NMD, implying that mRNAs bearing uORFs cannot generally be considered to represent NMD targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.