ArticleMolecular microbiology2006
Helical distribution of the bacterial chemoreceptor via colocalization with the Sec protein translocation machinery.
Article in Molecular microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 111 citations in OpenAlex.
- Enzymatic Labeling of Bacterial Proteins for Super-resolution Imaging in Live Cells.ACS central science · 2019Article
- A stochastic model of ion channel cluster formation in the plasma membrane.The Journal of general physiology · 2019Article
- Membrane Curvature and the Tol-Pal Complex Determine Polar Localization of the Chemoreceptor Tar in Escherichia coli.Journal of bacteriology · 2018Article
- Asymmetric polar localization dynamics of the serine chemoreceptor protein Tsr in Escherichia coli.PloS one · 2018Article
- The bacterial Sec system is required for the organization and function of the MreB cytoskeleton.PLoS genetics · 2017Article
- TatA complexes exhibit a marked change in organisation in response to expression of the TatBC complex.The Biochemical journal · 2017Article
- Molecular Interaction and Cellular Location of RecA and CheW Proteins inFrontiers in microbiology · 2016Article
- Dynamic Organization of SecA and SecY Secretion Complexes in the B. subtilis Membrane.PloS one · 2016Article
- Protein Connectivity in Chemotaxis Receptor Complexes.PLoS computational biology · 2015Article
- Transmembrane protein sorting driven by membrane curvature.Nature communications · 2015Article
- Breaking the bacterial protein targeting and translocation model: oral organisms as a case in point.Molecular oral microbiology · 2015Review
- The membrane: transertion as an organizing principle in membrane heterogeneity.Frontiers in microbiology · 2015Review
- Polar localization of Escherichia coli chemoreceptors requires an intact Tol-Pal complex.Molecular microbiology · 2014Article
- ParP prevents dissociation of CheA from chemotactic signaling arrays and tethers them to a polar anchor.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- SecYEG activates GTPases to drive the completion of cotranslational protein targeting.The Journal of cell biology · 2013Article
- Differential localization of the streptococcal accessory sec components and implications for substrate export.Journal of bacteriology · 2013Article
- The price of tags in protein localization studies.Journal of bacteriology · 2012Article
- Synergies between RNA degradation and trans-translation in Streptococcus pneumoniae: cross regulation and co-transcription of RNase R and SmpB.BMC microbiology · 2012Article
- Functional characterization and localization of a Bacillus subtilis sortase and its substrate and use of this sortase system to covalently anchor a heterologous protein to the B. subtilis cell wall for surface display.Journal of bacteriology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Escherichia coli, chemoreceptor clustering at a cell pole seems critical for signal amplification and adaptation. However, little is known about the mechanism of localization itself. Here we examined whether the aspartate chemoreceptor (Tar) is inserted directly into the polar membrane by using its fusion to green fluorescent protein (GFP). After induction of Tar-GFP, fluorescent spots first appeared in lateral membrane regions, and later cell poles became predominantly fluorescent. Unexpectedly, Tar-GFP showed a helical arrangement in lateral regions, which was more apparent when a Tar-GFP derivative with two cysteine residues in the periplasmic domain was cross-linked to form higher oligomers. Moreover, similar distribution was observed even when the cytoplasmic domain of the double cysteine Tar-GFP mutant was replaced by that of the kinase EnvZ, which does not localize to a pole. Observation of GFP-SecE and a translocation-defective MalE-GFP mutant, as well as indirect immunofluorescence microscopy on SecG, suggested that the general protein translocation machinery (Sec) itself is arranged into a helical array, with which Tar is transiently associated. The Sec coil appeared distinct from the MreB coil, an actin-like cytoskeleton. These findings will shed new light on the mechanisms underlying spatial organization of membrane proteins in E. coli.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.