ArticleProceedings of the National Academy of Sciences of the United States of America2006
The bundling activity of vasodilator-stimulated phosphoprotein is required for filopodium formation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
78 citing papers in PubMed, 158 citations in OpenAlex.
- Ovarian germline stem cell dedifferentiation is cytoneme dependent.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Rac1-PAK1-Arp2/3 signaling axis regulates CHIKV nsP1-induced filopodia and optimal viral genome replication.Journal of virology · 2024Article
- Liquid-like condensates mediate competition between actin branching and bundling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Role of Vasodilator-stimulated Phosphoproteins in the Development of Malignant Tumors.Current cancer drug targets · 2024Review
- Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping protein.Nature communications · 2023Article
- Liquid-like condensates mediate competition between actin branching and bundling.bioRxiv : the preprint server for biology · 2023Article
- Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping protein.bioRxiv : the preprint server for biology · 2023Article
- VASP localization to lipid bilayers induces polymerization driven actin bundle formation.Molecular biology of the cell · 2022Article
- Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation.Cell systems · 2022Article
- Article
- EVL regulates VEGF receptor-2 internalization and signaling in developmental angiogenesis.EMBO reports · 2021Article
- Formins specify membrane patterns generated by propagating actin waves.Molecular biology of the cell · 2020Article
- Ena orchestrates remodelling within the actin cytoskeleton to drive robustJournal of cell science · 2019Article
- Roles for Ena/VASP proteins in FMNL3-mediated filopodial assembly.Journal of cell science · 2018Article
- What Defines the "Kingdom" Fungi?Microbiology spectrum · 2017Review
- Interleukin-17 augments tumor necrosis factor α-mediated increase of hypoxia-inducible factor-1α and inhibits vasodilator-stimulated phosphoprotein expression to reduce the adhesion of breast cancer cells.Oncology letters · 2017Article
- Ena/VASP proteins regulate activated T-cell trafficking by promoting diapedesis during transendothelial migration.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration.Scientific reports · 2016Article
- Unravelling the Actin Cytoskeleton: A New Competitive Edge?Trends in cell biology · 2016Review
- De novo actin polymerization is required for model Hirano body formation in Dictyostelium.Biology open · 2016Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filopodia are highly dynamic finger-like cell protrusions filled with parallel bundles of actin filaments. Previously we have shown that Diaphanous-related formin dDia2 is involved in the formation of filopodia. Another key player for the formation of filopodia across many species is vasodilator-stimulated phosphoprotein (VASP). It has been proposed that the essential role of VASP for formation of filopodia is its competition with capping proteins for filament barbed-end interaction. To better understand the function of VASP in filopodium formation, we analyzed the in vitro and in vivo properties of Dictyostelium VASP (DdVASP) and extended our findings to human VASP. Recombinant VASP from both species nucleated and bundled actin filaments, but did not compete with capping proteins or block depolymerization from barbed ends. Together with the finding that DdVASP binds to the FH2 domain of dDia2, these data indicate that the crucial role of VASP in filopodium formation is different from uncapping of actin filaments. To identify the activity of DdVASP required in this process, rescue experiments of DdVASP-null cells with mutant DdVASP constructs were performed. Only WT DdVASP, but not a mutant lacking the F-actin bundling activity, could rescue the ability of these cells to form WT-like filopodia. Our data suggest that DdVASP is complexed with dDia2 in filopodial tips and support formin-mediated filament elongation by bundling nascent actin filaments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.