ArticleMolecular endocrinology (Baltimore, Md.)2006
Luteinizing hormone-induced RUNX1 regulates the expression of genes in granulosa cells of rat periovulatory follicles.
Article in Molecular endocrinology (Baltimore, Md.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 75 citations in OpenAlex.
- PGR-mediated transcriptome identifies SOX9 as a critical regulator of progesterone production in human ovulatory follicles.iScience · 2026Article
- New insights into the ovulatory process in the human ovary.Human reproduction update · 2025Review
- RUN(X) out of blood: emerging RUNX1 functions beyond hematopoiesis and links to Down syndrome.Human genomics · 2023Review
- Progesterone receptor mediates ovulatory transcription through RUNX transcription factor interactions and chromatin remodelling.Nucleic acids research · 2023Article
- An ex vivo ovulation system enables the discovery of novel ovulatory pathways and nonhormonal contraceptive candidates†.Biology of reproduction · 2023Article
- Article
- Neurotensin expression, regulation, and function during the ovulatory period in the mouse ovary†.Biology of reproduction · 2023Article
- Large-scale DNA demethylation occurs in proliferating ovarian granulosa cells during mouse follicular development.Communications biology · 2021Article
- The FOS/AP-1 Regulates Metabolic Changes and Cholesterol Synthesis in Human Periovulatory Granulosa Cells.Endocrinology · 2021Article
- Androgen-induced epigenetic modulations in the ovary.The Journal of endocrinology · 2021Review
- Androgen and Luteinizing Hormone Stimulate the Function of Rat Immature Leydig Cells Through Different Transcription Signals.Frontiers in endocrinology · 2021Article
- Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice.Scientific reports · 2020Article
- Activation of Steroidogenesis, Anti-Apoptotic Activity, and Proliferation in Porcine Granulosa Cells byGenes · 2020Article
- RUNX3 Promotes the Tumorigenic Phenotype in KGN, a Human Granulosa Cell Tumor-Derived Cell Line.International journal of molecular sciences · 2019Article
- Ovulation: Parallels With Inflammatory Processes.Endocrine reviews · 2019Review
- Ovulatory Induction of SCG2 in Human, Nonhuman Primate, and Rodent Granulosa Cells Stimulates Ovarian Angiogenesis.Endocrinology · 2018Article
- Core Binding Factor β Expression in Ovarian Granulosa Cells Is Essential for Female Fertility.Endocrinology · 2018Article
- Article
- Androgens Regulate Ovarian Gene Expression Through Modulation of Ezh2 Expression and Activity.Endocrinology · 2017Article
- Transcriptomic signatures for ovulation in vertebrates.General and comparative endocrinology · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
The LH surge induces specific transcription factors that regulate the expression of a myriad of genes in periovulatory follicles to bring about ovulation and luteinization. The present study determined 1) the localization of RUNX1, a nuclear transcription factor, 2) regulation of Runx1 mRNA expression, and 3) its potential function in rat ovaries. Up-regulation of mRNA and protein for RUNX1 is detected in preovulatory follicles after human chorionic gonadotropin (hCG) injection in gonadotropin-treated immature rats as well as after the LH surge in cycling animals by in situ hybridization and immunohistochemical and Western blot analyses. The regulation of Runx1 mRNA expression was investigated in vitro using granulosa cells from rat preovulatory ovaries. Treatments with hCG, forskolin, or phorbol 12 myristate 13-acetate stimulated Runx1 mRNA expression. The effects of hCG were reduced by inhibitors of protein kinase A, MAPK kinase, or p38 kinase, indicating that Runx1 expression is regulated by the LH-initiated activation of these signaling mediators. In addition, hCG-induced Runx1 mRNA expression was inhibited by a progesterone receptor antagonist and an epidermal growth factor receptor tyrosine kinase inhibitor, whereas amphiregulin stimulated Runx1 mRNA expression, demonstrating that the expression is mediated by the activation of the progesterone receptor and epidermal growth factor receptor. Finally, knockdown of Runx1 mRNA by small interfering RNA decreased progesterone secretion and reduced levels of mRNA for Cyp11a1, Hapln1, Mt1a, and Rgc32. The hormonally regulated expression of Runx1 in periovulatory follicles, its involvement in progesterone production, and regulation of preovulatory gene expression suggest important roles of RUNX1 in the periovulatory process.
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