ArticleApplied and environmental microbiology2006
Extracellular production of neoculin, a sweet-tasting heterodimeric protein with taste-modifying activity, by Aspergillus oryzae.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 75 citations in OpenAlex.
- Microbial Production of Alternative Proteins for Food Applications: Advances, Industrial Challenges, and Regulatory Status.Comprehensive reviews in food science and food safety · 2026Review
- Two-Step Engineering of Food-GradeJournal of fungi (Basel, Switzerland) · 2026Article
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Enhancement of Fungal Enzyme Production by Radio-Frequency Electromagnetic Fields.Journal of fungi (Basel, Switzerland) · 2022Article
- Recent progress in the use of diaziridine-based sweetener derivatives to elucidate the chemoreception mechanism of the sweet taste receptor.RSC advances · 2021Review
- Genome Editing Technology and Its Application Potentials in the Industrial Filamentous FungusJournal of fungi (Basel, Switzerland) · 2021Review
- The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool.Bioresources and bioprocessing · 2021Review
- Article
- Plasma-mediated enhancement of enzyme secretion in Aspergillus oryzae.Microbial biotechnology · 2021Article
- A Novel Potent Carrier for Unconventional Protein Export inFrontiers in cell and developmental biology · 2021Article
- Functional production of human antibody by the filamentous fungusFungal biology and biotechnology · 2020Article
- Functional Genomics ofMicroorganisms · 2019Review
- Bioproduction of the Recombinant Sweet Protein Thaumatin: Current State of the Art and Perspectives.Frontiers in microbiology · 2019Review
- Modulating endoplasmic reticulum-Golgi cargo receptors for improving secretion of carrier-fused heterologous proteins in the filamentous fungus Aspergillus oryzae.Applied and environmental microbiology · 2015Article
- Presence and functionality of mating type genes in the supposedly asexual filamentous fungus Aspergillus oryzae.Applied and environmental microbiology · 2012Article
- Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein.PloS one · 2011Article
- Enhanced production and secretion of heterologous proteins by the filamentous fungus Aspergillus oryzae via disruption of vacuolar protein sorting receptor gene Aovps10.Applied and environmental microbiology · 2010Article
- Expression of a synthetic Artemesia annua amorphadiene synthase in Aspergillus nidulans yields altered product distribution.Journal of industrial microbiology & biotechnology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neoculin (NCL), a protein with sweetness approximately 500-fold that of sugar, can be utilized as a nonglycemic sweetener. It also has taste-modifying activity to convert sourness to sweetness. NCL is a heterodimer composed of an N-glycosylated acidic subunit (NAS) and a basic subunit (NBS), which are conjugated by disulfide bonds. For the production of recombinant NCL (rNCL) by Aspergillus oryzae, alpha-amylase with a KEX2 cleavage site, -K-R-, was fused upstream of each of NAS and NBS and the resulting fusion proteins were simultaneously expressed. For accurate and efficient cleavage of the fusion construct by KEX2-like protease, a triglycine motif was inserted after the KEX2 cleavage site. As NBS showed lower production efficiency than did NAS, a larger amount of the NBS expression plasmid than of NAS expression plasmid was introduced during cotransformation, resulting in successful production of rNCL in the culture medium. Moreover, to obtain a higher production yield of rNCL, the active form of hacA cDNA encoding a transcription factor that induces an unfolded protein response was cloned and expressed constitutively. This resulted in a 1.5-fold increase in the level of rNCL production (2.0 mg/liter). rNCL was purified by chromatography, and its NAS was found to be N-glycosylated as expected. The original sweetness and taste-modifying activity of rNCL were comparable to those of native NCL when confirmed by calcium imaging with human embryonic kidney cells expressing the human sweet taste receptor and by sensory tests.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.