ArticleApplied and environmental microbiology2006
Use of fluorescence resonance energy transfer for rapid detection of enteroviral infection in vivo.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Antiviral candidates for enterovirus 71: targeting viral proteome and stage-specific lifecycle interventions.Virology journal · 2025Review
- Cell Cultures for Virology: Usability, Advantages, and Prospects.International journal of molecular sciences · 2020Review
- "Second-generation" fluorogenic RNA-based sensors.Methods (San Diego, Calif.) · 2019Review
- Dynamic protein assembly by programmable DNA strand displacement.Nature chemistry · 2018Article
- Rapid Determination of Ebolavirus Infectivity in Clinical Samples Using a Novel Reporter Cell Line.The Journal of infectious diseases · 2017Article
- A Novel Strategy for Live Detection of Viral Infection in Drosophila melanogaster.Scientific reports · 2016Article
- A Cell-based Fluorescence Resonance Energy Transfer (FRET) Sensor Reveals Inter- and Intragenogroup Variations in Norovirus Protease Activity and Polyprotein Cleavage.The Journal of biological chemistry · 2015Article
- Biochemical characterization of recombinant Enterovirus 71 3C protease with fluorogenic model peptide substrates and development of a biochemical assay.Antimicrobial agents and chemotherapy · 2015Article
- Article
- Inhibition of Enterovirus 71 replication by 7-hydroxyflavone and diisopropyl-flavon7-yl Phosphate.PloS one · 2014Article
- Detection and quantification of poliovirus infection using FTIR spectroscopy and cell culture.Journal of biological engineering · 2011Article
- Methods to detect infectious human enteric viruses in environmental water samples.International journal of hygiene and environmental health · 2011Review
- Evolution of cyclic peptide protease inhibitors.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Crystal structure of human enterovirus 71 3C protease.Journal of molecular biology · 2011Article
- Detection of infective poliovirus by a simple, rapid, and sensitive flow cytometry method based on fluorescence resonance energy transfer technology.Applied and environmental microbiology · 2010Article
- Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer.Antimicrobial agents and chemotherapy · 2009Article
- Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay.Antiviral research · 2008Article
- Design and application of highly responsive fluorescence resonance energy transfer biosensors for detection of sugar in living Saccharomyces cerevisiae cells.Applied and environmental microbiology · 2007Article
- Comparison of a reporter assay and immunomagnetic separation real-time reverse transcription-PCR for the detection of enteroviruses in seeded environmental water samples.Applied and environmental microbiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enteroviruses can be easily transmitted through the fecal-oral route and cause a diverse array of clinical manifestations. Recent outbreaks associated with enteroviral contamination in aquatic environments have called for the development of a more efficient and accurate virus monitoring system. To develop a simple, rapid, and direct method for identifying enteroviral infections, we generated a fluorescent reporter system in which genetically engineered cells express a hybrid fluorescent indicator composed of a linker peptide, which is exclusively cleaved by the 2A protease (2A(pro)), flanked with a cyan fluorescent protein (CFP) and a yellow fluorescent protein undergoing fluorescence resonance energy transfer. The covalent linkage between two fluorophores is disrupted due to 2A(pro) activity upon viral infection, which results in an increase in CFP intensity. This allows the rapid (within 7.5 h) detection of very low numbers (10 PFU or fewer) of infectious enteroviruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.