ArticleApplied and environmental microbiology2006
RNA-based stable isotope probing and isolation of anaerobic benzene-degrading bacteria from gasoline-contaminated groundwater.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 182 citations in OpenAlex.
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- Evaluation of Biodegradation of BTEX in the Subsurface of a Petrochemical Site near the Yangtze River, China.International journal of environmental research and public health · 2022Article
- Assessing the Biodegradation of BTEX and Stress Response in a Bio-Permeable Reactive Barrier Using Compound-Specific Isotope Analysis.International journal of environmental research and public health · 2022Article
- Anaerobic benzene mineralization by natural microbial communities from Niger Delta.Biodegradation · 2021Article
- Optimized Cultivation and Syntrophic Relationship of Anaerobic Benzene-Degrading Enrichment Cultures under Methanogenic Conditions.Microbes and environments · 2021Article
- Simulating metagenomic stable isotope probing datasets with MetaSIPSim.BMC bioinformatics · 2020Article
- Complete Genome Sequence of an Anaerobic Benzene-Degrading Bacterium,Microbiology resource announcements · 2019Article
- Anaerobic Benzene Mineralization by Nitrate-Reducing and Sulfate-Reducing Microbial Consortia Enriched From the Same Site: Comparison of Community Composition and Degradation Characteristics.Microbial ecology · 2018Article
- A benzene-degrading nitrate-reducing microbial consortium displays aerobic and anaerobic benzene degradation pathways.Scientific reports · 2018Article
- Benzene degradation in a denitrifying biofilm reactor: activity and microbial community composition.Applied microbiology and biotechnology · 2017Article
- Benzene Degradation by a Variovorax Species within a Coal Tar-Contaminated Groundwater Microbial Community.Applied and environmental microbiology · 2017Article
- The ecology of anaerobic degraders of BTEX hydrocarbons in aquifers.FEMS microbiology ecology · 2017Article
- Integrated Anaerobic-Aerobic Biodegradation of Multiple Contaminants Including Chlorinated Ethylenes, Benzene, Toluene, and Dichloromethane.Water, air, and soil pollution · 2017Article
- Polycyclic Aromatic Hydrocarbon-Induced Changes in Bacterial Community Structure under Anoxic Nitrate Reducing Conditions.Frontiers in microbiology · 2016Article
- Indoor Heating Drives Water Bacterial Growth and Community Metabolic Profile Changes in Building Tap Pipes during the Winter Season.International journal of environmental research and public health · 2015Article
- Characterization of para-Nitrophenol-Degrading Bacterial Communities in River Water by Using Functional Markers and Stable Isotope Probing.Applied and environmental microbiology · 2015Article
- Association of Growth Substrates and Bacterial Genera with Benzo[Environmental engineering science · 2014Article
- Metatranscriptome of an anaerobic benzene-degrading, nitrate-reducing enrichment culture reveals involvement of carboxylation in benzene ring activation.Applied and environmental microbiology · 2014Article
- Time-resolved DNA stable isotope probing links Desulfobacterales- and Coriobacteriaceae-related bacteria to anaerobic degradation of benzene under methanogenic conditions.Microbes and environments · 2014Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable isotope probing (SIP) of benzene-degrading bacteria in gasoline-contaminated groundwater was coupled to denaturing gradient gel electrophoresis (DGGE) of DNA fragments amplified by reverse transcription-PCR from community 16S rRNA molecules. Supplementation of the groundwater with [(13)C(6)]benzene together with an electron acceptor (nitrate, sulfate, or oxygen) showed that a phylotype affiliated with the genus Azoarcus specifically appeared in the (13)C-RNA fraction only when nitrate was supplemented. This phylotype was also observed as the major band in DGGE analysis of bacterial 16S rRNA gene fragments amplified by PCR from the gasoline-contaminated groundwater. In order to isolate the Azoarcus strains, the groundwater sample was streaked on agar plates containing nonselective diluted CGY medium, and the DGGE analysis was used to screen colonies formed on the plates. This procedure identified five bacterial isolates (from 60 colonies) that corresponded to the SIP-identified Azoarcus phylotype, among which two strains (designated DN11 and AN9) degraded benzene under denitrifying conditions. Incubation of these strains with [(14)C]benzene showed that the labeled carbon was mostly incorporated into (14)CO(2) within 14 days. These results indicate that the Azoarcus population was involved in benzene degradation in the gasoline-contaminated groundwater under denitrifying conditions. We suggest that RNA-based SIP identification coupled to phylogenetic screening of nonselective isolates facilitates the isolation of enrichment/isolation-resistant microorganisms with a specific function.
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