ArticleApplied and environmental microbiology2006
Survival strategy of Erwinia amylovora against copper: induction of the viable-but-nonculturable state.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 129 citations in OpenAlex.
- Copper-induced stress mechanisms inApplied and environmental microbiology · 2025Article
- First EuropeanBiology · 2024Article
- Improved Canker Processing and Viability Droplet Digital PCR Allow Detection ofMicroorganisms · 2024Article
- Persistence and viable but non-culturable state induced by streptomycin inFrontiers in microbiology · 2024Article
- Antagonistic and protective activity of Lactobacillus plantarum strain 17 M against E. amylovora.World journal of microbiology & biotechnology · 2023Article
- Axenic Long-Term Cultivation ofJournal of fungi (Basel, Switzerland) · 2023Article
- Transcriptional profiling of Xanthomonas campestris pv. campestris in viable but nonculturable state.BMC genomics · 2023Article
- Wake Up! Resuscitation of Viable but Nonculturable Bacteria: Mechanism and Potential Application.Foods (Basel, Switzerland) · 2022Review
- Article
- Control ofPlants (Basel, Switzerland) · 2022Article
- Fire blight resistance, irrigation and conducive wet weather improveFrontiers in microbiology · 2022Article
- The Role of RelA and SpoT on ppGpp Production, Stress Response, Growth Regulation, and Pathogenicity in Xanthomonas campestris pv.Microbiology spectrum · 2021Article
- A Bifunctional Peptide Conjugate That Controls Infections ofMolecules (Basel, Switzerland) · 2021Article
- Persister Cells Form in the Plant PathogenMicroorganisms · 2021Article
- Characteristics of the copper-induced viable-but-non-culturable state in bacteria.World journal of microbiology & biotechnology · 2021Review
- Survey of Toxin⁻Antitoxin Systems inToxins · 2019Article
- Induction and Resuscitation of the Viable but Non-culturable (VBNC) State inFrontiers in microbiology · 2019Article
- Detection of Clavibacter michiganensis subsp. michiganensis in viable but nonculturable state from tomato seed using improved qPCR.PloS one · 2018Article
- Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene.Molecular plant pathology · 2018Article
- Persistence in Phytopathogenic Bacteria: Do We Know Enough?Frontiers in microbiology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Copper compounds, widely used to control plant-pathogenic bacteria, have traditionally been employed against fire blight, caused by Erwinia amylovora. However, recent studies have shown that some phytopathogenic bacteria enter into the viable-but-nonculturable (VBNC) state in the presence of copper. To determine whether copper kills E. amylovora or induces the VBNC state, a mineral medium without copper or supplemented with 0.005, 0.01, or 0.05 mM Cu(2+) was inoculated with 10(7) CFU/ml of this bacterium and monitored over 9 months. Total and viable cell counts were determined by epifluorescence microscopy using the LIVE/DEAD kit and by flow cytometry with 5-cyano-2,3-ditolyl tetrazolium chloride and SYTO 13. Culturable cells were counted on King's B nonselective solid medium. Changes in the bacterial morphology in the presence of copper were observed by scanning electron microscopy. E. amylovora entered into the VBNC state at all three copper concentrations assayed, much faster when the copper concentration increased. The addition of different agents which complex copper allowed the resuscitation (restoration of culturability) of copper-induced VBNC cells. Finally, copper-induced VBNC cells were virulent only for the first 5 days, while resuscitated cells always regained their pathogenicity on immature fruits over 9 months. These results have shown, for the first time, the induction of the VBNC state in E. amylovora as a survival strategy against copper.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.