ArticleApplied and environmental microbiology2006
Engineering of a xylose metabolic pathway in Corynebacterium glutamicum.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 239 citations in OpenAlex.
- Draft genome and physiological characterization of a newly isolated L-arabinose-utilizing Corynebacterium glutamicum CS176.Scientific reports · 2026Article
- Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain ofSynthetic and systems biotechnology · 2025Article
- Single Mutation inMicroorganisms · 2025Article
- Unsilencing a cryptic xylose metabolic pathway in Rhodococcus jostii RHA1 for efficient lipid production from lignocellulosic biomass.Journal of biological engineering · 2025Article
- EngineeringBiodesign research · 2025Review
- Metabolic Engineering ofMolecules (Basel, Switzerland) · 2024Article
- Review of the Proteomics and Metabolic Properties ofMicroorganisms · 2024Review
- Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate.Biotechnology for biofuels and bioproducts · 2023Article
- From Aquaculture to Aquaculture: Production of the Fish Feed Additive Astaxanthin byMolecules (Basel, Switzerland) · 2023Article
- Global Cellular Metabolic Rewiring Adapts Corynebacterium glutamicum to Efficient Nonnatural Xylose Utilization.Applied and environmental microbiology · 2022Article
- Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering.Metabolic engineering communications · 2022Review
- Application of Corynebacterium glutamicum engineering display system in three generations of biorefinery.Microbial cell factories · 2022Review
- Article
- Metabolic Engineering for Valorization of Agri- and Aqua-Culture Sidestreams for Production of Nitrogenous Compounds byFrontiers in microbiology · 2022Review
- Synthesis of aromatic amino acids from 2G lignocellulosic substrates.Microbial biotechnology · 2021Article
- Improved glucose and xylose co-utilization by overexpression of xylose isomerase and/or xylulokinase genes in oleaginous fungus Mucor circinelloides.Applied microbiology and biotechnology · 2021Article
- Sustainable Production ofMicroorganisms · 2021Article
- Exploring the Potential ofFrontiers in bioengineering and biotechnology · 2021Article
- Utilization of a Wheat Sidestream for 5-Aminovalerate Production inFrontiers in bioengineering and biotechnology · 2021Article
- Lactic acid production - producing microorganisms and substrates sources-state of art.Heliyon · 2020Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aerobic microorganism Corynebacterium glutamicum was metabolically engineered to broaden its substrate utilization range to include the pentose sugar xylose, which is commonly found in agricultural residues and other lignocellulosic biomass. We demonstrated the functionality of the corynebacterial xylB gene encoding xylulokinase and constructed two recombinant C. glutamicum strains capable of utilizing xylose by cloning the Escherichia coli gene xylA encoding xylose isomerase, either alone (strain CRX1) or in combination with the E. coli gene xylB (strain CRX2). These genes were provided on a high-copy-number plasmid and were under the control of the constitutive promoter trc derived from plasmid pTrc99A. Both recombinant strains were able to grow in mineral medium containing xylose as the sole carbon source, but strain CRX2 grew faster on xylose than strain CRX1. We previously reported the use of oxygen deprivation conditions to arrest cell replication in C. glutamicum and divert carbon source utilization towards product production rather than towards vegetative functions (M. Inui, S. Murakami, S. Okino, H. Kawaguchi, A. A. Vertès, and H. Yukawa, J. Mol. Microbiol. Biotechnol. 7:182-196, 2004). Under these conditions, strain CRX2 efficiently consumed xylose and produced predominantly lactic and succinic acids without growth. Moreover, in mineral medium containing a sugar mixture of 5% glucose and 2.5% xylose, oxygen-deprived strain CRX2 cells simultaneously consumed both sugars, demonstrating the absence of diauxic phenomena relative to the new xylA-xylB construct, albeit glucose-mediated regulation still exerted a measurable influence on xylose consumption kinetics.
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