ArticleApplied and environmental microbiology2006
Genomic and proteomic analyses of the agarolytic system expressed by Saccharophagus degradans 2-40.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 118 citations in OpenAlex.
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- Mining Arylsulfatase from Genome-Scale Metabolic Pathways of Pseudoalteromonas sp. SR43-6 and Its Agar-Based Desulfurization Applications.ACS omega · 2025Article
- Characterization of a novel β-agarase from Antarctic macroalgae-associated bacteria metagenomic library and anti-inflammatory activity of the enzymatic hydrolysates.Frontiers in microbiology · 2022Article
- Development of a Novel Cell Surface Attachment System to Display Multi-Protein Complex Using the Cohesin-Dockerin Binding Pair.Journal of microbiology and biotechnology · 2021Article
- A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3Applied and environmental microbiology · 2021Article
- Article
- The structure of PfGH50B, an agarase from the marine bacterium Pseudoalteromonas fuliginea PS47.Acta crystallographica. Section F, Structural biology communications · 2020Article
- Dual Agarolytic Pathways in a Marine Bacterium,Applied and environmental microbiology · 2020Article
- Comparative Genomic and Secretomic Analysis Provide Insights Into Unique Agar Degradation Function of Marine BacteriumFrontiers in microbiology · 2020Article
- Genome-Wide Identification and Functional Characterization of β-Agarases inFrontiers in microbiology · 2020Article
- Characterization of a Novel α-Neoagarobiose Hydrolase Capable of Preparation of Medium- and Long-Chain Agarooligosaccharides.Frontiers in bioengineering and biotechnology · 2019Article
- Recombinant β-agarases: insights into molecular, biochemical, and physiochemical characteristics.3 Biotech · 2018Review
- Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont.Nature communications · 2018Article
- Elevated temperature drives kelp microbiome dysbiosis, while elevated carbon dioxide induces water microbiome disruption.PloS one · 2018Article
- Genomic diversification of giant enteric symbionts reflects host dietary lifestyles.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production.Bioengineering (Basel, Switzerland) · 2017Review
- Agarolytic bacterium Persicobacter sp. CCB-QB2 exhibited a diauxic growth involving galactose utilization pathway.MicrobiologyOpen · 2017Article
- Horizontal Transfer of a Novel Soil Agarase Gene from Marine Bacteria to Soil Bacteria via Human Microbiota.Scientific reports · 2016Article
- Biochemical and Structural Characterization of a Five-domain GH115 α-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T.The Journal of biological chemistry · 2016Article
- A laboratory case study of efficient polyhydoxyalkonates production by Bacillus cereus, a contaminant in Saccharophagus degradans ATCC 43961 in minimal sea salt media.Current microbiology · 2014Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saccharophagus degradans 2-40 (formerly Microbulbifer degradans 2-40) is a marine gamma-subgroup proteobacterium capable of degrading many complex polysaccharides, such as agar. While several agarolytic systems have been characterized biochemically, the genetics of agarolytic systems have been only partially determined. By use of genomic, proteomic, and genetic approaches, the components of the S. degradans 2-40 agarolytic system were identified. Five agarases were identified in the S. degradans 2-40 genome. Aga50A and Aga50D include GH50 domains. Aga86C and Aga86E contain GH86 domains, whereas Aga16B carries a GH16 domain. Novel family 6 carbohydrate binding modules (CBM6) were identified in Aga16B and Aga86E. Aga86C has an amino-terminal acylation site, suggesting that it is surface associated. Aga16B, Aga86C, and Aga86E were detected by mass spectrometry in agarolytic fractions obtained from culture filtrates of agar-grown cells. Deletion analysis revealed that aga50A and aga86E were essential for the metabolism of agarose. Aga16B was shown to endolytically degrade agarose to release neoagarotetraose, similarly to a beta-agarase I, whereas Aga86E was demonstrated to exolytically degrade agarose to form neoagarobiose. The agarolytic system of S. degradans 2-40 is thus predicted to be composed of a secreted endo-acting GH16-dependent depolymerase, a surface-associated GH50-dependent depolymerase, an exo-acting GH86-dependent agarase, and an alpha-neoagarobiose hydrolase to release galactose from agarose.
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