ArticleApplied and environmental microbiology2006
Stolbur phytoplasma genome survey achieved using a suppression subtractive hybridization approach with high specificity.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 34 citations in OpenAlex.
- The genome of Candidatus phytoplasma ziziphi provides insights into their biological characteristics.BMC plant biology · 2023Article
- Multilocus Sequence Analysis Reveals Three Distinct Populations of "Applied and environmental microbiology · 2019Article
- An Effective Pipeline Based on Relative Coverage for the Genome Assembly of Phytoplasmas and Other Fastidious Prokaryotes.Current genomics · 2018Article
- 'Candidatus Phytoplasma phoenicium' associated with almond witches'-broom disease: from draft genome to genetic diversity among strain populations.BMC microbiology · 2015Article
- Impact of vector dispersal and host-plant fidelity on the dissemination of an emerging plant pathogen.PloS one · 2012Article
- Identification of genes differentially expressed during interaction of Mexican lime tree infected with "Candidatus Phytoplasma aurantifolia".BMC microbiology · 2011Article
- Striking diversity of vmp1, a variable gene encoding a putative membrane protein of the stolbur phytoplasma.Applied and environmental microbiology · 2009Article
- Ancient, recurrent phage attacks and recombination shaped dynamic sequence-variable mosaics at the root of phytoplasma genome evolution.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Multilocus sequence typing confirms the close genetic interrelatedness of three distinct flavescence dorée phytoplasma strain clusters and group 16SrV phytoplasmas infecting grapevine and alder in Europe.Applied and environmental microbiology · 2007Article
- Optimizing Phytoplasma DNA purification for genome analysis.Journal of biomolecular techniques : JBT · 2007Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Phytoplasmas are unculturable bacterial plant pathogens transmitted by phloem-feeding hemipteran insects. DNA of phytoplasmas is difficult to purify because of their exclusive phloem location and low abundance in plants. To overcome this constraint, suppression subtractive hybridization (SSH) was modified and used to selectively amplify DNA of the stolbur phytoplasma infecting a periwinkle plant. Plasmid libraries were constructed, and the origins of the DNA inserts were verified by hybridization and PCR screenings. After a single round of SSH, there was still a significant level of contamination with plant DNA (around 50%). However, the modified SSH, which included a second round of subtraction (double SSH), resulted in an increased phytoplasma DNA purity (97%). Results validated double SSH as an efficient way to produce a genome survey for microbial agents unavailable in culture. Assembly of 266 insert sequences revealed 181 phytoplasma genetic loci which were annotated. Comparative analysis of 113 kbp indicated that among 217 protein coding sequences, 83% were homologous to "Candidatus Phytoplasma asteris" (OY-M strain) genes, with hits widely distributed along the chromosome. Most of the stolbur-specific SSH sequences were orphan genes, with the exception of two partial coding sequences encoding proteins homologous to a mycoplasma surface protein and riboflavin kinase.
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