ArticleJournal of clinical microbiology2006
Comparison of DNA fingerprinting methods for use in investigation of type E botulism outbreaks in the Canadian Arctic.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Identification of a newFrontiers in microbiology · 2024Article
- Draft Genome Sequences of Two Clostridium botulinum Group II Strains Carrying Phage-Like Plasmids.Microbiology resource announcements · 2022Article
- Pulsotype Diversity of Clostridium botulinum Strains Containing Serotypes A and/or B Genes.Foodborne pathogens and disease · 2017Article
- Tracking sources of Clostridium botulinum type E contamination in seal meat.International journal of circumpolar health · 2017Article
- Clostridium botulinum Group II Isolate Phylogenomic Profiling Using Whole-Genome Sequence Data.Applied and environmental microbiology · 2015Article
- Molecular gene profiling of Clostridium botulinum group III and its detection in naturally contaminated samples originating from various European countries.Applied and environmental microbiology · 2015Article
- Identification and genetic characterization of Clostridium botulinum serotype A strains from commercially pasteurized carrot juice.Food microbiology · 2014Article
- Two novel toxin variants revealed by whole-genome sequencing of 175 Clostridium botulinum type E strains.Applied and environmental microbiology · 2014Article
- BEC, a novel enterotoxin of Clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks.Infection and immunity · 2014Article
- Genetic diversity of the flagellin genes of Clostridium botulinum groups I and II.Applied and environmental microbiology · 2013Article
- Genomic and physiological variability within Group II (non-proteolytic) Clostridium botulinum.BMC genomics · 2013Article
- Distribution of Clostridium botulinum type E strains in Nunavik, Northern Quebec, Canada.Applied and environmental microbiology · 2013Article
- Article
- Phosphorothioate DNA as an antioxidant in bacteria.Nucleic acids research · 2012Article
- Intestinal toxemia botulism in 3 adults, Ontario, Canada, 2006-2008.Emerging infectious diseases · 2012Article
- Analysis of Clostridium botulinum serotype E strains by using multilocus sequence typing, amplified fragment length polymorphism, variable-number tandem-repeat analysis, and botulinum neurotoxin gene sequencing.Applied and environmental microbiology · 2011Article
- In vitro detection and quantification of botulinum neurotoxin type e activity in avian blood.Applied and environmental microbiology · 2011Article
- Biodiversity of Clostridium botulinum type E associated with a large outbreak of botulism in wildlife from Lake Erie and Lake Ontario.Applied and environmental microbiology · 2011Article
- Development and application of a new method for specific and sensitive enumeration of spores of nonproteolytic Clostridium botulinum types B, E, and F in foods and food materials.Applied and environmental microbiology · 2010Article
- Susceptibility of Vibrio parahaemolyticus to tris-dependent DNA degradation during pulsed-field gel electrophoresis.Journal of clinical microbiology · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) analysis, and automated ribotyping were compared for epidemiological typing of Clostridium botulinum type E using clinical and food isolates associated with four botulism outbreaks occurring in the Canadian Arctic. All type E strains previously untypeable by PFGE, even with the use of a formaldehyde fixation step, could be typed by the addition of 50 microM thiourea to the electrophoresis running buffer. Digestion with SmaI or XhoI followed by PFGE was used to link food and clinical isolates from four different type E botulism outbreaks and differentiate them from among 39 group II strains. Strain differentiation was unsuccessful with the automated ribotyping system, producing a single characteristic EcoRI fingerprint common to all group II strains. RAPD analysis of C. botulinum group II strains was not consistently reproducible with primer OPJ-6 or OPJ-13, apparently discriminating between epidemiologically related strains. A modified PFGE protocol was judged to be the most useful method for typing epidemiologically related C. botulinum type E strains, based on its ability to type all strains reproducibly and with an adequate level of discrimination.
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