ArticleMolecular biology of the cell2006
Dynein-dependent motility of microtubules and nucleation sites supports polarization of the tubulin array in the fungus Ustilago maydis.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Nuclear division phenotypes in Sporidiobolales and Trichosporonales.Microbiology spectrum · 2025Article
- Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes.Journal of fungi (Basel, Switzerland) · 2021Article
- Nuclear migration in budding yeasts: position before division.Current genetics · 2019Review
- Cytoplasmic Transport Machinery of the SPF27 Homologue Num1 in Ustilago maydis.Scientific reports · 2018Article
- Microtubule Motors in Establishment of Epithelial Cell Polarity.Cold Spring Harbor perspectives in biology · 2018Review
- Cell Biology of Hyphal Growth.Microbiology spectrum · 2017Review
- Active diffusion and microtubule-based transport oppose myosin forces to position organelles in cells.Nature communications · 2016Article
- Article
- Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2015Article
- A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation.eLife · 2015Article
- Article
- Motor-driven motility of fungal nuclear pores organizes chromosomes and fosters nucleocytoplasmic transport.The Journal of cell biology · 2012Article
- Dancing genomes: fungal nuclear positioning.Nature reviews. Microbiology · 2009Review
- The machinery for cell polarity, cell morphogenesis, and the cytoskeleton in the Basidiomycete fungus Ustilago maydis-a survey of the genome sequence.Fungal genetics and biology : FG & B · 2008Review
- Dynamic rearrangement of nucleoporins during fungal "open" mitosis.Molecular biology of the cell · 2008Article
- Hyphal growth: a tale of motors, lipids, and the Spitzenkörper.Eukaryotic cell · 2007Review
- Pathocycles: Ustilago maydis as a model to study the relationships between cell cycle and virulence in pathogenic fungi.Molecular genetics and genomics : MGG · 2006Review
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules (MTs) are often organized by a nucleus-associated MT organizing center (MTOC). In addition, in neurons and epithelial cells, motor-based transport of assembled MTs determines the polarity of the MT array. Here, we show that MT motility participates in MT organization in the fungus Ustilago maydis. In budding cells, most MTs are nucleated by three to six small and motile gamma-tubulin-containing MTOCs at the boundary of mother and daughter cell, which results in a polarized MT array. In addition, free MTs and MTOCs move rapidly throughout the cytoplasm. Disruption of MTs with benomyl and subsequent washout led to an equal distribution of the MTOC and random formation of highly motile and randomly oriented MTs throughout the cytoplasm. Within 3 min after washout, MTOCs returned to the neck region and the polarized MT array was reestablished. MT motility and polarity of the MT array was lost in dynein mutants, indicating that dynein-based transport of MTs and MTOCs polarizes the MT cytoskeleton. Observation of green fluorescent protein-tagged dynein indicated that this is achieved by off-loading dynein from the plus-ends of motile MTs. We propose that MT organization in U. maydis involves dynein-mediated motility of MTs and nucleation sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.