ArticleNucleic acids research2006
The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- MAP30 transgenic tobacco lines: from silencing to inducing.Molecular biology reports · 2021Article
- DNA demethylase ROS1 prevents inheritable DREB1A/CBF3 repression by transgene-induced promoter methylation in the Arabidopsis ice1-1 mutant.Plant molecular biology · 2020Article
- Genome and transcriptome characterization of the glycoengineered Nicotiana benthamiana line ΔXT/FT.BMC genomics · 2019Article
- Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing.BMC plant biology · 2018Article
- Effect of orientation of transcription of a gene in an inverted transferred DNA repeat on transcriptional gene silencing in rice transgenics-a case study.Physiology and molecular biology of plants : an international journal of functional plant biology · 2015Article
- Reconstructing de novo silencing of an active plant retrotransposon.Nature genetics · 2013Article
- Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants.BMC plant biology · 2013Article
- Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture.Epigenetics · 2013Article
- Low relative humidity triggers RNA-directed de novo DNA methylation and suppression of genes controlling stomatal development.Journal of experimental botany · 2012Article
- Paramutation of tobacco transgenes by small RNA-mediated transcriptional gene silencing.Epigenetics · 2011Article
- Hairpin transcription does not necessarily lead to efficient triggering of the RNAi pathway.Transgenic research · 2011Article
- Faithful inheritance of cytosine methylation patterns in repeated sequences of the allotetraploid tobacco correlates with the expression of DNA methyltransferase gene families from both parental genomes.Molecular genetics and genomics : MGG · 2009Article
- Cell culture-induced gradual and frequent epigenetic reprogramming of invertedly repeated tobacco transgene epialleles.Plant physiology · 2009Article
- Enzyme-Linked Electrochemical Detection of PCR-Amplified Nucleotide Sequences Using Disposable Screen-Printed Sensors. Applications in Gene Expression Monitoring.Sensors (Basel, Switzerland) · 2008Article
- Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system.Plant physiology · 2007Article
- Low abundant spacer 5S rRNA transcripts are frequently polyadenylated in Nicotiana.Molecular genetics and genomics : MGG · 2007Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the in trans-silencing capacities of a transgene locus that carried the neomycin phosphotransferase II reporter gene linked to the 35S promoter in an inverted repeat (IR). This transgene locus was originally posttranscriptionally silenced but switched to a transcriptionally silenced epiallele after in vitro tissue culture. Here, we show that both epialleles were strongly methylated in the coding region and IR center. However, by genomic sequencing, we found that the 1.0 kb region around the transcription start site was heavily methylated in symmetrical and non-symmetrical contexts in transcriptionally but not in posttranscriptionally silenced epilallele. Also, the posttranscriptionally silenced epiallele could trans-silence and trans-methylate homologous transgene loci irrespective of their genomic organization. We demonstrate that this in trans-silencing was accompanied by the production of small RNA molecules. On the other hand, the transcriptionally silenced variant could neither trans-silence nor trans-methylate homologous sequences, even after being in the same genetic background for generations and meiotic cycles. Interestingly, 5-aza-2-deoxy-cytidine-induced hypomethylation could partially restore signaling from the transcriptionally silenced epiallele. These results are consistent with the hypothesis that non-transcribed highly methylated IRs are poor silencers of homologous loci at non-allelic positions even across two generations and that transcription of the inverted sequences is essential for their trans-silencing potential.
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