ArticleNucleic acids research2006
Hairpin-induced tRNA-mediated (HITME) recombination in HIV-1.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 18 citations in OpenAlex.
- t-RNA mediates provirus deletion in HIV-infected cells.Retrovirology · 2025Article
- Extinction of all infectious HIV in cell culture by the CRISPR-Cas12a system with only a single crRNA.Nucleic acids research · 2020Article
- Influence of a 3' Terminal Ribozyme on AgoshRNA Biogenesis and Activity.Molecular therapy. Nucleic acids · 2019Article
- Silencing of HIV-1 by AgoshRNA molecules.Gene therapy · 2017Article
- CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Towards Antiviral shRNAs Based on the AgoshRNA Design.PloS one · 2015Article
- The impact of unprotected T cells in RNAi-based gene therapy for HIV-AIDS.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- Anticipating and blocking HIV-1 escape by second generation antiviral shRNAs.Retrovirology · 2010Article
- Combinatorial RNAi against HIV-1 using extended short hairpin RNAs.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- The remarkable frequency of human immunodeficiency virus type 1 genetic recombination.Microbiology and molecular biology reviews : MMBR · 2009Review
- Human immunodeficiency virus type 1 escape is restricted when conserved genome sequences are targeted by RNA interference.Journal of virology · 2008Article
- Effects of identity minimization on Moloney murine leukemia virus template recognition and frequent tertiary template-directed insertions during nonhomologous recombination.Journal of virology · 2007Article
- Environment determines fidelity for an RNA virus replicase.Journal of virology · 2007Article
- Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome.Retrovirology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombination due to template switching during reverse transcription is a major source of genetic variability in retroviruses. In the present study we forced a recombination event in human immunodeficiency virus type 1 (HIV-1) by electroporation of T cells with DNA from a molecular HIV-1 clone that has a 300 bp long hairpin structure in the Nef gene (HIV-lhNef). HIV-lhNef does not replicate, but replication-competent escape variants emerged in four independent cultures. The major part of the hairpin was deleted in all escape viruses. In three cases, the hairpin deletion was linked to patch insertion of tRNA(asp), tRNA(glu) or tRNA(trp) sequences. The tRNAs were inserted in the viral genome in the antisense orientation, indicating that tRNA-mediated recombination occurred during minus-strand DNA synthesis. We here propose a mechanistic model for this hairpin-induced tRNA-mediated (HITME) recombination. The transient role of the cellular tRNA molecule as enhancer of retroviral recombination is illustrated by the eventual removal of inserted tRNA sequences by a subsequent recombination/deletion event.
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