ArticlePlant physiology1992
Contribution of Malate and Amino Acid Metabolism to Cytoplasmic pH Regulation in Hypoxic Maize Root Tips Studied Using Nuclear Magnetic Resonance Spectroscopy.
Article in Plant physiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 138 citations in OpenAlex.
- Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots.Antioxidants (Basel, Switzerland) · 2022Article
- Complex Gene Regulation Underlying Mineral Nutrient Homeostasis in Soybean Root Response to Acidity Stress.Genes · 2019Article
- Nitrate nutrition influences multiple factors in order to increase energy efficiency under hypoxia in Arabidopsis.Annals of botany · 2019Article
- Seed Dormancy Involves a Transcriptional Program That Supports Early Plastid Functionality during Imbibition.Plants (Basel, Switzerland) · 2018Article
- Functional Conservation and Divergence of Soybean GmSTOP1 Members in Proton and Aluminum Tolerance.Frontiers in plant science · 2018Article
- Metabolic and physiological adjustment of Suaeda maritima to combined salinity and hypoxia.Annals of botany · 2017Article
- Proteins involved in biophoton emission and flooding-stress responses in soybean under light and dark conditions.Molecular biology reports · 2016Article
- Regulation of Primary Metabolism in Response to Low Oxygen Availability as Revealed by Carbon and Nitrogen Isotope Redistribution.Plant physiology · 2016Article
- Ectopic expression of CaRLK1 enhances hypoxia tolerance with increasing alanine production in Nicotiana spp.Plant molecular biology · 2014Article
- Tolerance of anaerobic conditions caused by flooding during germination and early growth in rice (Oryza sativa L.).Frontiers in plant science · 2013Article
- Differential regulation of alanine aminotransferase homologues by abiotic stresses in wheat (Triticum aestivum L.) seedlings.Plant cell reports · 2012Article
- High phenotypic plasticity of Suaeda maritima observed under hypoxic conditions in relation to its physiological basis.Annals of botany · 2012Article
- pH regulation in anoxic rice coleoptiles at pH 3.5: biochemical pHstats and net H+ influx in the absence and presence of NOFormula.Journal of experimental botany · 2012Article
- Role of plant glyoxylate reductases during stress: a hypothesis.The Biochemical journal · 2009Review
- Erythrina speciosa (Leguminosae-Papilionoideae) under soil water saturation: morphophysiological and growth responses.Annals of botany · 2009Article
- STOP1 regulates multiple genes that protect arabidopsis from proton and aluminum toxicities.Plant physiology · 2009Article
- Plant mitochondrial function during anaerobiosis.Annals of botany · 2009Review
- Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots.Plant molecular biology · 2008Article
- Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions.Plant physiology · 2008Article
- Nitrite reduces cytoplasmic acidosis under anoxia.Plant physiology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
(31)P-, (13)C-, and (15)N-nuclear magnetic resonance spectroscopy were used to determine the roles of malate, succinate, Ala, Asp, Glu, Gln, and gamma-aminobutyrate (GABA) in the energy metabolism and regulation of cytoplasmic pH in hypoxic maize (Zea mays L.) root tips. Nitrogen status was manipulated by perfusing root tips with ammonium sulfate prior to hypoxia; this pretreatment led to enhanced synthesis of Ala early in hypoxia, and of GABA at later times. We show that: (a) the ability to regulate cytoplasmic pH during hypoxia is not significantly affected by enhanced Ala synthesis. (b) Independent of nitrogen status, decarboxylation of Glu to GABA is greatest after several hours of hypoxia, as metabolism collapses. (c) Early in hypoxia, cytoplasmic malate is in part decarboxylated to pyruvate (leading to Ala, lactate, and ethanol), and in part converted to succinate. It appears that activation of malic enzyme serves to limit cytoplasmic acidosis early in hypoxia. (d) Ala synthesis in hypoxic root tips under these conditions is due to transfer of nitrogen ultimately derived from Asp and Gln, present in oxygenated tissue. We describe the relative contributions of glycolysis and malate decarboxylation in providing Ala carbons. (e) Succinate accumulation during hypoxia can be attributed to metabolism of Asp and malate; this flux to succinate is energetically negligible. There is no detectable net flux from Glc to succinate during hypoxia. The significance of the above metabolic reactions relative to ethanol and lactate production, and to flooding tolerance, is discussed. The regulation of the patterns of metabolism during hypoxia is considered with respect to cytoplasmic pH and redox state.
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Registered trials
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