ArticlePlant physiology1991
Microtubules in mesophyll cells of nonacclimated and cold-acclimated spinach : visualization and responses to freezing, low temperature, and dehydration.
Article in Plant physiology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 58 citations in OpenAlex.
- Hydrogen Peroxide Response in Leaves of Poplar (Populus simonii × Populus nigra) Revealed from Physiological and Proteomic Analyses.International journal of molecular sciences · 2017Article
- Is lithium biologically an important or toxic element to living organisms? An overview.Environmental science and pollution research international · 2017Review
- Analysis of the Prefoldin Gene Family in 14 Plant Species.Frontiers in plant science · 2016Article
- Microtubules and the tax payer.Protoplasma · 2012Review
- Cold stability of microtubules in wood-forming tissues of conifers during seasons of active and dormant cambium.Planta · 2012Article
- Cell physiology of plants growing in cold environments.Protoplasma · 2010Review
- The root microtubule cytoskeleton and cell cycle analysis through desiccation of Brassica napus seedlings.Protoplasma · 2008Article
- Microtubules are a target for self-incompatibility signaling in Papaver pollen.Plant physiology · 2008Article
- ARP2 and ARP3 are localized to sites of actin filament nucleation in tobacco BY-2 cells.Protoplasma · 2006Article
- Chilling stability of microtubules in root-tip cells of cucumber.Plant cell reports · 2003Article
- Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not.Plant physiology · 2000Article
- Induction of cold stability of microtubules in cultured tobacco cells.Plant physiology · 1992Article
- Lithium decreases cold-induced microtubule depolymerization in mesophyll cells of spinach.Plant physiology · 1992Article
- Effect of microtubule stabilization on the freezing tolerance of mesophyll cells of spinach.Plant physiology · 1991Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Responses of cortical microtubules in spinach (Spinacia oleracea L. cv Bloomsdale) mesophyll cells to freezing, thawing, supercooling, and dehydration were assessed. Microtubules were visualized using a modified procedure for indirect immunofluorescence microscopy. Leaf sections of nonacclimated and cold-acclimated spinach were slowly frozen to various temperatures, fixed while frozen, and microtubules immunolabelled. Both nonacclimated and cold-acclimated cells exhibited nearly complete microtubule depolymerization after ice formation. After 1 hour thawing at 23 degrees C, microtubules in both nonacclimated and cold-acclimated cells repolymerized. With time, however, microtubules in nonacclimated cells again depolymerized. Since microtubules in cells of leaf tissue frozen slowly are subjected to dehydration as well as subzero temperatures, these stresses were applied separately and their effects on microtubules noted. Supercooling induced microtubule depolymerization in both nonacclimated and cold-acclimated cells, but to a smaller extent than did freezing. Exposing leaf sections to solutions of sorbitol (a cell wall-penetrating osmoticum) or polyethylene glycol 10,000 (a nonpenetrating osmoticum) at room temperature caused microtubule depolymerization. The effects of low temperature and dehydration are roughly additive in producing the observed microtubule responses during freezing. Only small differences in microtubule stability were resolved between nonacclimated and cold-acclimated cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.