ArticlePlant physiology1990
Tonoplast ion channels from sugar beet cell suspensions : inhibition by amiloride and its analogs.
Article in Plant physiology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 17 citations in OpenAlex.
- The sugar beet gene encoding the sodium/proton exchanger 1 (BvNHX1) is regulated by a MYB transcription factor.Planta · 2010Article
- Characterization of Vacuolar Malate and K Channels under Physiological Conditions.Plant physiology · 1992Article
- Cytoplasmic chloride regulates cation channels in the vacuolar membrane of plant cells.The Journal of membrane biology · 1992Article
- Identification of a 170-kDa protein associated with the vacuolar Na+/H+ antiport of Beta vulgaris.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The properties of the vacuolar membrane (tonoplast) ion channels of sugar beet (Beta vulgaries) cell cultures were studied using the patch-clamp technique. Tonoplast currents displayed inward rectification in the whole vacuole and isolated outside-out patch configurations and permeability ratios P(K+)/P(Na+) = 1 and P(K+)/P(Cl-) = 5. Amiloride and two of its analogs, 5-(N-methyl-N-isobutyl)-amiloride and benzamil, inhibitors of Na(+) channels in animal systems, blocked inward currents by reducing single-channel openings. Concentrations for 50% inhibition of vacuolar currents of 730 nanomolar, 130 nanomolar, and 1.5 micromolar for amiloride, benzamil, and 5-(N-methyl-N-isobutyl)-amiloride, respectively, were obtained from whole-vacuole recordings. The high inhibitory action (affinity) of amiloride and its analogs for the tonoplast cation channel suggests that these compounds could be used for the isolation and biochemical characterization of this protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.