ArticlePlant physiology1987
Intracellular localization of heat shock proteins in maize.
Article in Plant physiology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 69 citations in OpenAlex.
- Integrated analysis of HSP20 genes in the developing flesh of peach: identification, expression profiling, and subcellular localization.BMC plant biology · 2023Article
- Molecular chaperone activity of tomato (Lycopersicon esculentum) endoplasmic reticulum-located small heat shock protein.Journal of plant research · 2008Article
- Accumulation of small heat-shock protein homologs in the endoplasmic reticulum of cortical parenchyma cells in mulberry in association with seasonal cold acclimation.Plant physiology · 1999Article
- Heat-stress response of maize mitochondria.Plant physiology · 1998Article
- The 23-kDa light-stress-regulated heat-shock protein of chenopodium rubrum L. is located in the mitochondria.Planta · 1997Article
- Molecular characterization of cDNAs encoding low-molecular-weight heat shock proteins of soybean.Plant molecular biology · 1996Article
- An endomembrane-localized small heat-shock protein from Arabidopsis thaliana.Plant physiology · 1995Article
- A Low Molecular Mass Heat-Shock Protein Is Localized to Higher Plant Mitochondria.Plant physiology · 1994Article
- Localization of small heat shock proteins to the higher plant endomembrane system.Molecular and cellular biology · 1993Article
- Genotype-specific heat shock proteins in two maize inbreds.Plant cell reports · 1992Article
- Temporal and spatial expression patterns of the small heat shock (hsp16) genes in transgenic Caenorhabditis elegans.Molecular biology of the cell · 1992Article
- Effect of temperature conditioning on chilling injury of cucumber cotyledons: possible role of abscisic Acid and heat shock proteins.Plant physiology · 1991Article
- Synthesis of early heat shock proteins in young leaves of barley and sorghum.Plant physiology · 1990Article
- Cloning, sequence analysis, and expression of a cDNA encoding a plastid-localized heat shock protein in maize.Plant physiology · 1990Article
- Heat Shock Inhibits alpha-Amylase Synthesis in Barley Aleurone without Inhibiting the Activity of Endoplasmic Reticulum Marker Enzymes.Plant physiology · 1990Article
- Heat shock protein synthesis and thermal tolerance in wheat.Plant physiology · 1989Article
- The effects of salt stress on polypeptides in membrane fractions from barley roots.Plant physiology · 1988Article
- Dynamic changes in the structure and intracellular locale of the mammalian low-molecular-weight heat shock protein.Molecular and cellular biology · 1988Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intracellular distribution of the maize root heat shock proteins (hsp) was studied as a step toward understanding their physiological function. Linear sucrose density centrifugation was employed to separate organelles so the relative quantities of hsp in different subcellular compartments could be analyzed in a single preparation. Gradient fractions were assayed for the presence of hsp by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and for marker enzyme activities. Analyses of 15 to 60% gradients showed five hsp to be organelle associated. Hsp 25 and 72 were in fractions containing closely equilibrating Golgi and endoplasmic reticulum marker activities, while hsp 18, 29, and 72 were in fractions containing overlapping plasma membrane, mitochondria, and glyoxysomal marker activities. Hsp larger than 72 kilodaltons were not present in gradient fractions. A second fractionation scheme achieved better separation of the two sets of closely equilibrating organelles. When a 13,000g centrifugation step to remove mitochondria was employed prior to gradient centrifugation, hsp 29 was absent from the gradient fractions. If the buoyant density of the endoplasmic reticulum was shifted by either maintaining the ribosomes on the membrane or removing them, a corresponding shift in the equilibrium positions of hsp 25 and 72 occurred. Hsp 18 and 70 remained in plasma membrane-containing fractions irrespective of these treatments.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.