ArticlePlant physiology1992
Tonoplast-bound protein kinase phosphorylates tonoplast intrinsic protein.
Article in Plant physiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 94 citations in OpenAlex.
- Hormonal and environmental signaling pathways target membrane water transport.Plant physiology · 2021Review
- The roles of selectivity filters in determining aluminum transport by AtNIP1;2.Plant signaling & behavior · 2021Article
- Phosphorylation of TIP3 Aquaporins duringCells · 2019Article
- Flowers under pressure: ins and outs of turgor regulation in development.Annals of botany · 2014Review
- Plant aquaporins with non-aqua functions: deciphering the signature sequences.Plant molecular biology · 2011Article
- Ser123 is essential for the water channel activity of McPIP2;1 from Mesembryanthemum crystallinum.The Journal of biological chemistry · 2010Article
- The PIP and TIP aquaporins in wheat form a large and diverse family with unique gene structures and functionally important features.Functional & integrative genomics · 2008Article
- Analysis of EF-hand-containing proteins in Arabidopsis.Genome biology · 2002Article
- The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants.Plant physiology · 2001Article
- Lentil seed aquaporins form a hetero-oligomer which is phosphorylated by a Mg(2+)-dependent and Ca(2+)-regulated kinase.The Biochemical journal · 2000Article
- Rice calcium-dependent protein kinase isoforms OsCDPK2 and OsCDPK11 show different responses to light and different expression patterns during seed development.Plant molecular biology · 1999Article
- Proteins for transport of water and mineral nutrients across the membranes of plant cells.The Plant cell · 1999Review
- Protein storage bodies and vacuolesThe Plant cell · 1999Article
- Osmotic water permeability of isolated protoplasts. Modifications during developmentPlant physiology · 1999Article
- Water transport activity of the plasma membrane aquaporin PM28A is regulated by phosphorylation.The Plant cell · 1998Article
- A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.The EMBO journal · 1996Article
- Characterization of two integral membrane proteins located in the protein bodies of pumpkin seeds.Plant molecular biology · 1995Article
- Phosphorylation regulates the water channel activity of the seed-specific aquaporin alpha-TIP.The EMBO journal · 1995Article
- Two genes that encode Ca(2+)-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana.Molecular & general genetics : MGG · 1994Article
- The Expression Pattern of the Tonoplast Intrinsic Protein gamma-TIP in Arabidopsis thaliana Is Correlated with Cell Enlargement.Plant physiology · 1992Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tonoplast intrinsic protein (TIP) is a member of a family of putative membrane channels found in bacteria, animals, and plants. Plants have seed-specific, vegetative/reproductive organ-specific, and water-stress-induced forms of TIP. Here, we report that the seed-specific TIP is a phosphoprotein whose phosphorylation can be monitored in vivo by allowing bean cotyledons to take up [(32)P]orthophosphate and in vitro by incubating purified tonoplasts with gamma-labeled [(32)P]ATP. Characterization of the in vitro phosphorylation of TIP indicates that a membrane-bound protein kinase phosphorylates TIP in a Ca(2+)-dependent manner. The capacity of the isolated tonoplast membranes to phosphorylate TIP declined markedly during seed germination, and this decline occurred well before the development-mediated decrease in TIP occurs. Phosphoamino acid analysis of purified, radiolabeled TIP showed that serine is the major, if not only, phosphorylated residue, and cyanogen bromide cleavage yielded a single radioactive peptide peak on a reverse-phase high-performance liquid chromatogram. Estimation of the molecular mass of the cyanogen bromide phosphopeptide by laser desorption mass spectroscopy led to its identification as the hydrophilic N-terminal domain of TIP. The putative phosphate-accepting serine residue occurs in a consensus phosphorylation site for serine/threonine protein kinases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.