ArticleGenes & development2006
A mitotic topoisomerase II checkpoint in budding yeast is required for genome stability but acts independently of Pds1/securin.
Article in Genes & development, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Quantifying complexity in DNA structures with high resolution Atomic Force Microscopy.Nature communications · 2025Article
- Yeast Tools for Studying Type II Topoisomerases in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cell cycle responses to Topoisomerase II inhibition: Molecular mechanisms and clinical implications.The Journal of cell biology · 2023Review
- Topoisomerase II deficiency leads to a postreplicative structural shift in all Saccharomyces cerevisiae chromosomes.Scientific reports · 2021Article
- Role of Aurora B and Haspin kinases in the metaphase Topoisomerase II checkpoint.Cell cycle (Georgetown, Tex.) · 2021Review
- Topoisomerase II SUMOylation activates a metaphase checkpoint via Haspin and Aurora B kinases.The Journal of cell biology · 2020Article
- Article
- Review
- A Topology-Centric View on Mitotic Chromosome Architecture.International journal of molecular sciences · 2017Review
- Non-Catalytic Roles of the Topoisomerase IIα C-Terminal Domain.International journal of molecular sciences · 2017Review
- Genome-Scale Genetic Interactions and Cell Imaging Confirm Cytokinesis as Deleterious to Transient Topoisomerase II Deficiency inG3 (Bethesda, Md.) · 2017Article
- A noncatalytic function of the topoisomerase II CTD in Aurora B recruitment to inner centromeres during mitosis.The Journal of cell biology · 2016Article
- Mitotic catenation is monitored and resolved by a PKCε-regulated pathway.Nature communications · 2014Article
- Defective histone supply causes condensin-dependent chromatin alterations, SAC activation and chromosome decatenation impairment.Nucleic acids research · 2014Article
- The NIMA kinase is required to execute stage-specific mitotic functions after initiation of mitosis.Eukaryotic cell · 2014Article
- Direct monitoring of the strand passage reaction of DNA topoisomerase II triggers checkpoint activation.PLoS genetics · 2013Article
- Article
- DNA topoisomerase II and its growing repertoire of biological functions.Nature reviews. Cancer · 2009Review
- Review
- The why and how of DNA unlinking.Nucleic acids research · 2009Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Topoisomerase II (Topo II) performs topological modifications on double-stranded DNA molecules that are essential for chromosome condensation, resolution, and segregation. In mammals, G2 and metaphase cell cycle delays induced by Topo II poisons have been proposed to be the result of checkpoint activation in response to the catenation state of DNA. However, the apparent lack of such controls in model organisms has excluded genetic proof that Topo II checkpoints exist and are separable from the conventional DNA damage checkpoint controls. But here, we define a Topo II-dependent G2/M checkpoint in a genetically amenable eukaryote, budding yeast, and demonstrate that this checkpoint enhances cell survival. Conversely, a lack of the checkpoint results in aneuploidy. Neither DNA damage-responsive pathways nor Pds1/securin are needed for this checkpoint. Unusually, spindle assembly checkpoint components are required for the Topo II checkpoint, but checkpoint activation is not the result of failed chromosome biorientation or a lack of spindle tension. Thus, compromised Topo II function activates a yeast checkpoint system that operates by a novel mechanism.
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Registered trials
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