ArticleThe American journal of pathology2006
Doxycycline alters vascular smooth muscle cell adhesion, migration, and reorganization of fibrillar collagen matrices.
Article in The American journal of pathology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 101 citations in OpenAlex.
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- Lymphatic Malformations: Review of Diagnosis and Management for the Interventional Radiologist.Seminars in interventional radiology · 2024Review
- EDI3 knockdown in ER-HER2+ breast cancer cells reduces tumor burden and improves survival in two mouse models of experimental metastasis.Breast cancer research : BCR · 2024Article
- Inhibition of Metalloproteinases Extends Longevity and Function of In Vitro Human iPSC-Derived Skeletal Muscle.Biomedicines · 2024Article
- A Pulsatile Flow System to Engineer Aneurysm and Atherosclerosis Mimetic Extracellular Matrix.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Doxycycline-Eluting Core-Shell Type Nanofiber-Covered Trachea Stent for Inhibition of Cellular Metalloproteinase and Its Related Fibrotic Stenosis.Pharmaceutics · 2019Article
- Matrix remodeling in chronic lung diseases.Matrix biology : journal of the International Society for Matrix Biology · 2018Review
- Multifunctional, JNK-inhibiting nanotherapeutics for augmented elastic matrix regenerative repair in aortic aneurysms.Drug delivery and translational research · 2018Article
- COMPARISON OF ANTI BACTERIAL EFFICACY OF PHOTODYNAMIC THERAPY AND DOXYCYCLINE ON AGGREGATIBACTER ACTINOMYCETEMCOMITANS.African journal of infectious diseases · 2018Article
- Assessment of solid microneedle rollers to enhance transmembrane delivery of doxycycline and inhibition of MMP activity.Drug delivery · 2017Article
- Matrix Metalloproteinases -14, -9 and -2 are Localized to the Podosome and Involved in Podosome Development in the A7r5 Smooth Muscle Cell.Journal of cardiobiology · 2017Article
- Cathepsin K-targeted sub-micron particles for regenerative repair of vascular elastic matrix.Acta biomaterialia · 2017Article
- Nanoparticulate delivery of agents for induced elastogenesis in three-dimensional collagenous matrices.Journal of tissue engineering and regenerative medicine · 2016Article
- Improving the stability of chitosan-gelatin-based hydrogels for cell delivery using transglutaminase and controlled release of doxycycline.Drug delivery and translational research · 2015Article
- ZIPK is critical for the motility and contractility of VSMCs through the regulation of nonmuscle myosin II isoforms.American journal of physiology. Heart and circulatory physiology · 2014Article
- Fibulin-3, -4, and -5 are highly susceptible to proteolysis, interact with cells and heparin, and form multimers.The Journal of biological chemistry · 2013Article
- Doxycycline prevents matrix remodeling and contraction by trichiasis-derived conjunctival fibroblasts.Investigative ophthalmology & visual science · 2013Article
- Perspectives on stem cell-based elastic matrix regenerative therapies for abdominal aortic aneurysms.Stem cells translational medicine · 2013Article
- Multifunctional nanoparticles for doxycycline delivery towards localized elastic matrix stabilization and regenerative repair.Acta biomaterialia · 2013Article
- Decrease of MMP-9 activity improves soleus muscle regeneration.Tissue engineering. Part A · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Remodeling of injured blood vessels is dependent on smooth muscle cells and matrix metalloproteinase activity. Doxycycline is a broad spectrum matrix metalloproteinase inhibitor that is under investigation for the treatment of acute coronary syndromes and aneurysms. In the present study, we examine the mechanisms by which doxycycline inhibits smooth muscle cell responses using a series of in vitro assays that mimic critical steps in pathological vascular remodeling. Doxycycline treatment dramatically increased smooth muscle cell adhesion to the substrate, as evidenced by interference reflection microscopy and immunostaining for paxillin and phosphotyrosine. Cell aggregation was also potentiated after treatment with doxycycline. Treatment with 104 mumol/L doxycycline reduced thymidine uptake by 58% compared with untreated cells (P < 0.05) and inhibited closure of a scrape wound made in a smooth muscle cell monolayer by 20% (P < 0.05). Contraction of a three-dimensional collagen gel was used as an in vitro model for constrictive vessel remodeling, demonstrating that treatment with 416 mumol/L doxycycline for 12 hours inhibited collagen gel remodeling by 37% relative to control (P < 0.05). In conclusion, we have shown that doxycycline treatment leads to dramatically increased smooth muscle cell adhesion, which in turn might limit responses in pathological vascular remodeling.
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