ArticleJournal of clinical pathology1991
Episomal and integrated human papillomavirus in cervical neoplasia shown by non-isotopic in situ hybridisation.
Article in Journal of clinical pathology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 169 citations in OpenAlex.
- The Diagnostic Value of Circulating Cell-Free HPV DNA in Plasma from Cervical Cancer Patients.Cells · 2022Article
- Genome-wide profiling of human papillomavirus DNA integration in liquid-based cytology specimens from a Gabonese female population using HPV capture technology.Scientific reports · 2019Article
- Detection of HPV16 in Esophageal Cancer in a High-Incidence Region of Malawi.International journal of molecular sciences · 2018Article
- Review
- Integration of the full-length HPV16 genome in cervical cancer and Caski and Siha cell lines and the possible ways of HPV integration.Virus genes · 2015Article
- Evidence that alpha-9 human papillomavirus infections are a major etiologic factor for oropharyngeal carcinoma in black South Africans.Head and neck pathology · 2013Article
- Evaluation of a combined triple method to detect causative HPV in oral and oropharyngeal squamous cell carcinomas: p16 Immunohistochemistry, Consensus PCR HPV-DNA, and In Situ Hybridization.Infectious agents and cancer · 2012Article
- Discrimination of 'driver' and 'passenger' HPV in tonsillar carcinomas by the polymerase chain reaction, chromogenic in situ hybridization, and p16(INK4a) immunohistochemistry.Head and neck pathology · 2011Article
- Significance of p53-binding protein 1 nuclear foci in uterine cervical lesions: endogenous DNA double strand breaks and genomic instability during carcinogenesis.Histopathology · 2011Article
- Human papillomavirus (HPV) detection using in situ hybridization in histologic samples: correlations with cytologic changes and polymerase chain reaction HPV detection.American journal of clinical pathology · 2011Article
- Mechanism of genomic instability in cells infected with the high-risk human papillomaviruses.PLoS pathogens · 2009Article
- Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer.Virchows Archiv : an international journal of pathology · 2008Article
- Evaluation of a commercialized in situ hybridization assay for detecting human papillomavirus DNA in tissue specimens from patients with cervical intraepithelial neoplasia and cervical carcinoma.Journal of clinical microbiology · 2008Article
- Human papillomavirus DNA and e6/e7 mRNA status in relation to survival of patients treated for cervical squamous cell carcinoma.The open virology journal · 2008Article
- Genomic instability of the host cell induced by the human papillomavirus replication machinery.The EMBO journal · 2007Article
- Morules in endometrial carcinoma and benign endometrial lesions differ from squamous differentiation tissue and are not infected with human papillomavirus.Journal of clinical pathology · 2004Article
- Comparison of real-time PCR signal-amplified in situ hybridization and conventional PCR for detection and quantification of human papillomavirus in archival cervical cancer tissue.Journal of clinical microbiology · 2004Article
- Review
- Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens.BMC clinical pathology · 2003Article
- Squamous metaplasia induced by transfection of human papillomavirus DNA into cultured adenocarcinoma cells.Molecular pathology : MP · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It was postulated that non-isotopic in situ hybridisation (NISH) signal types 1-3 for human papillomavirus in cervical biopsy specimens represent episomal or integrated virus. The aim of this study was to validate this hypothesis by independent molecular techniques. Fresh cervical intraepithelial neoplasia (CIN) and squamous cell cancer (SCC) tissue were examined for NISH signal pattern by hybridising with digoxigenin labelled HPV 16. DNA was extracted from the same samples and analysed by restriction endonuclease digestion and Southern blotting to determine the physical state of the viral genome. Six CIN biopsy specimens showed a type 1 NISH signal for HPV 16. On Southern analysis these biopsy specimens contained only episomal HPV 16. Three SCC with a type 2 NISH signal contained integrated HPV 16 by Southern analysis. Two specimens, a CIN 3 and an SCC with a type 3 NISH signal for HPV 16, showed the presence of both episomal and integrated HPV 16 with conventional Southern analysis and two dimensional gel electrophoresis. These results show that episomal HPV can be reliably determined by NISH type 1 signal, integrated HPV by type 2, and a combination of both episomal and integrated HPV, by a type 3 signal in archival paraffin wax embedded cervical biopsy specimens. This will add another variable to the epidemiological studies of HPV infection. In particular, it will now allow retrospective studies to be done to define the role of episomal and integrated HPV in the evolution of cervical intraepithelial neoplasia and other cervical disease associated with this virus.
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