ArticleLangmuir : the ACS journal of surfaces and colloids2006
Exploring the interaction of ruthenium(II) polypyridyl complexes with DNA using single-molecule techniques.
Article in Langmuir : the ACS journal of surfaces and colloids, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 103 citations in OpenAlex.
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- Protocellular energy transduction, information and fitness.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- Binuclear ruthenium complex linker length tunes DNA threading intercalation kinetics.Biophysical journal · 2025Article
- TMPyP binding evokes a complex, tunable nanomechanical response in DNA.Nucleic acids research · 2024Article
- Minimal Functionalization of Ruthenium Compounds with Enhanced Photoreactivity against Hard-to-Treat Cancer Cells and Resistant Bacteria.Inorganic chemistry · 2024Article
- Binding and stabilizating effect of RNA triplex poly(U)⋅poly(A)*poly(U) by enantiomers of ruthenium(II) polypyridyl complex [Ru(bpy)Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Article
- Left versus right: Exploring the effects of chiral threading intercalators using optical tweezers.Biophysical journal · 2022Article
- Molecular structure, DNA binding mode, photophysical properties and recommendations for use of SYBR Gold.Nucleic acids research · 2021Article
- Ratiometric Fluorescence Detection of DNA Based on the Inner Filter Effect of Ru(bpy)ACS omega · 2021Article
- Flexibility and thermal dynamic stability increase of dsDNA induced by Ru(bpy)BMC chemistry · 2019Article
- Article
- Recent Developments in the Interactions of Classic Intercalated Ruthenium Compounds: [Ru(bpy)₂dppz]Molecules (Basel, Switzerland) · 2019Review
- Mechanisms of small molecule-DNA interactions probed by single-molecule force spectroscopy.Nucleic acids research · 2016Article
- Dissecting the Dynamic Pathways of Stereoselective DNA Threading Intercalation.Biophysical journal · 2016Article
- A ruthenium dimer complex with a flexible linker slowly threads between DNA bases in two distinct steps.Nucleic acids research · 2015Article
- Strong DNA deformation required for extremely slow DNA threading intercalation by a binuclear ruthenium complex.Nucleic acids research · 2014Article
- Differential contribution of basic residues to HIV-1 nucleocapsid protein's nucleic acid chaperone function and retroviral replication.Nucleic acids research · 2014Article
- PT-ACRAMTU, a platinum-acridine anticancer agent, lengthens and aggregates, but does not stiffen or soften DNA.Cell biochemistry and biophysics · 2013Article
- Force spectroscopy reveals the DNA structural dynamics that govern the slow binding of Actinomycin D.Nucleic acids research · 2012Article
- Atomic force microscopy study of DNA conformation in the presence of drugs.European biophysics journal : EBJ · 2011Article
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7 authors at 3 institutions in 1 country.
Funding
Abstract
Here we explore DNA binding by a family of ruthenium(II) polypyridyl complexes using an atomic force microscope (AFM) and optical tweezers. We demonstrate using AFM that Ru(bpy)2dppz2+ intercalates into DNA (K(b) = 1.5 x 10(5) M(-1)), as does its close relative Ru(bpy)2dppx2+ (K(b) = 1.5 x 10(5) M(-1)). However, intercalation by Ru(phen)3(2+) and other Ru(II) complexes with K(b) values lower than that of Ru(bpy)2dppz2+ is difficult to determine using AFM because of competing aggregation and surface-binding phenomena. At the high Ru(II) concentrations required to evaluate intercalation, most of the DNA strands acquire a twisted, curled conformation that is impossible to measure accurately. The condensation of DNA on mica in the presence of polycations is well known, but it clearly precludes the accurate assessment by AFM of DNA intercalation by most Ru(II) complexes, though not by ethidium bromide and other monovalent intercalators. When stretching individual DNA molecules using optical tweezers, the same limitation on high metal concentration does not exist. Using optical tweezers, we show that Ru(phen)2dppz2+ intercalates avidly (K(b) = 3.2 x 10(6) M(-1)) whereas Ru(bpy)3(2+) does not intercalate, even at micromolar ruthenium concentrations. Ru(phen)3(2+) is shown to intercalate weakly (i.e., at micromolar concentrations (K(b) = 8.8 x 10(3) M(-1))). The distinct differences in DNA stretching behavior between Ru(phen)3(2+) and Ru(bpy)3(2+) clearly illustrate that intercalation can be distinguished from groove binding by pulling the DNA with optical tweezers. Our results demonstrate both the benefits and challenges of two single-molecule methods of exploring DNA binding and help to elucidate the mode of binding of Ru(phen)3(2+).
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