ArticlePflugers Archiv : European journal of physiology1991
Pharmacological block of Ca(2+)-activated Cl- current in rat vascular smooth muscle cells in short-term primary culture.
Article in Pflugers Archiv : European journal of physiology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Drug-Related Lymphedema: Mysteries, Mechanisms, and Potential Therapies.Frontiers in pharmacology · 2022Review
- TMEM16A in Cystic Fibrosis: Activating or Inhibiting?Frontiers in pharmacology · 2019Review
- A Calcium-Dependent Chloride Current Increases Repetitive Firing in Mouse Sympathetic Neurons.Frontiers in physiology · 2018Article
- Molecular and functional significance of Ca(2+)-activated Cl(-) channels in pulmonary arterial smooth muscle.Pulmonary circulation · 2015Review
- 9-Anthracene carboxylic acid is more suitable than DIDS for characterization of calcium-activated chloride current during canine ventricular action potential.Naunyn-Schmiedeberg's archives of pharmacology · 2015Article
- Human spermatozoa possess a calcium-dependent chloride channel that may participate in the acrosomal reaction.The Journal of physiology · 2012Article
- A Ca²⁺-dependent chloride current and Ca²⁺ influx via Ca(v)1.2 ion channels play major roles in P2Y receptor-mediated pulmonary vasoconstriction.British journal of pharmacology · 2012Article
- Ca2+-activated Cl- current in retinal arteriolar smooth muscle.Investigative ophthalmology & visual science · 2009Article
- Effects of calcium channel blockers on antidepressant action of Alprazolam and Imipramine.The Libyan journal of medicine · 2007Article
- Role of acid-sensitive outwardly rectifying anion channels in acidosis-induced cell death in human epithelial cells.Pflugers Archiv : European journal of physiology · 2007Article
- Diversity of Cl(-) channels.Cellular and molecular life sciences : CMLS · 2006Review
- Calcium and chloride channel activation by angiotensin II-AT1 receptors in preglomerular vascular smooth muscle cells.American journal of physiology. Renal physiology · 2005Article
- Calcium-activated chloride current in normal mouse sympathetic ganglion cells.The Journal of physiology · 1997Article
- Chloride ion efflux regulates adherence, spreading, and respiratory burst of neutrophils stimulated by tumor necrosis factor-alpha (TNF) on biologic surfaces.The Journal of cell biology · 1996Article
- Two distinct membrane currents activated by cyclopiazonic acid-induced calcium store depletion in single smooth muscle cells of the mouse anococcygeus.British journal of pharmacology · 1996Article
- Investigation of the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) on membrane currents in rat portal vein.British journal of pharmacology · 1996Article
- Differential beta-adrenergic regulation and phenotypic modulation of voltage-gated calcium currents in rat aortic myocytes.The Journal of physiology · 1994Article
- Photoreleased inositol 1,4,5-trisphosphate-induced response in single smooth muscle cells of rat portal vein.The Journal of physiology · 1994Article
- Action of niflumic acid on evoked and spontaneous calcium-activated chloride and potassium currents in smooth muscle cells from rabbit portal vein.British journal of pharmacology · 1994Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca(2+)-activated Cl- currents were studied in isolated cells from rat portal vein smooth muscle in short-term primary culture using the whole-cell patch-clamp technique. Cl- currents can be activated separately by Ca2+ release from intracellular stores (in response to external applications of caffeine or noradrenaline) and by Ca2+ influx through voltage-dependent Ca2+ channels. The effects of several Cl- channel blockers and of spironolactone (a substance known to reduce internal Ca2+ loading) on both Cl- and Ca2+ currents were examined. Diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), anthracene-9-carboxylic acid (9-AC) and diphenylamine-2,2'-dicarboxylic acid (DPC) inhibited the Ca(2+)-activated Cl- current (IC50 values between 16.5 and 306 microM) with no effects on the inward Ca2+ current and on internal Ca2+ loading (testing by measuring the Ca(2+)-activated K+ current). These results indicate that the inhibition of Cl- current by these compounds is due to a direct interaction with the Cl- channel. In contrast, spironolactone inhibited both K+ and Cl- currents (IC50 = 7.6 microM) by reducing the amount of Ca2+ located in the internal stores, whereas the Cl- current activated by Ca2+ current through T-type Ca2+ channels was unchanged. This preparation and the protocols developed in this study appears to be appropriate for analysis of substances interfering with Cl- channels or intracellular Ca2+ stores.
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