ArticlePlant molecular biology2006
Expression of a Brassica isopropylmalate synthase gene in Arabidopsis perturbs both glucosinolate and amino acid metabolism.
Article in Plant molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Review
- An agroecological structure model of compost-soil-plant interactions for sustainable organic farming.ISME communications · 2023Article
- Homeostasis of branched-chain amino acids is critical for the activity of TOR signaling ineLife · 2019Article
- Mutations in the Arabidopsis ROL17/isopropylmalate synthase 1 locus alter amino acid content, modify the TOR network, and suppress the root hair cell development mutant lrx1.Journal of experimental botany · 2019Article
- Influence of isopropylmalate synthase OsIPMS1 on seed vigour associated with amino acid and energy metabolism in rice.Plant biotechnology journal · 2019Article
- Comparative Transcriptome Analyses Reveal a Special Glucosinolate Metabolism Mechanism inFrontiers in plant science · 2016Article
- Transporters in plant sulfur metabolism.Frontiers in plant science · 2014Review
- Article
- Engineering synthetic recursive pathways to generate non-natural small molecules.Nature chemical biology · 2012Review
- Gene expression profiling of phytoplasma-infected Madagascar periwinkle leaves using differential display.Molecular biology reports · 2011Article
- From amino acid to glucosinolate biosynthesis: protein sequence changes in the evolution of methylthioalkylmalate synthase in Arabidopsis.The Plant cell · 2011Article
- Article
- Characterization of alpha-isopropylmalate synthases containing different copy numbers of tandem repeats in Mycobacterium tuberculosis.BMC microbiology · 2009Article
- Transcriptomic and reverse genetic analyses of branched-chain fatty acid and acyl sugar production in Solanum pennellii and Nicotiana benthamiana.Plant physiology · 2008Article
- Two Arabidopsis genes (IPMS1 and IPMS2) encode isopropylmalate synthase, the branchpoint step in the biosynthesis of leucine.Plant physiology · 2007Article
- Grouping Gene Ontology terms to improve the assessment of gene set enrichment in microarray data.BMC bioinformatics · 2006Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isopropylmalate synthase (IPMS) is a key enzyme in the biosynthesis of the essential amino acid leucine, and thus primary metabolism. In Arabidopsis, the functionally similar enzyme, methythiolalkylmalate synthase (MAM), is an important enzyme in the elongation of methionine prior to glucosinolate (GSL) biosynthesis, as part of secondary metabolism. We describe the cloning of an IPMS gene from Brassica, BatIMS, and its functional characterisation by heterologous expression in E. coli and Arabidopsis. Over expression of BatIMS in Arabidopsis resulted in plants with an aberrant phenotype, reminiscent of mutants in GSL biosynthesis. Metabolite analyses showed that these plants had both perturbed amino acid metabolism and enhanced levels of GSLs. Microarray profiling showed that BatIMS over expression caused up regulation of the genes for methionine-derived GSL biosynthesis, and down regulation of genes involved in leucine catabolism, in addition to perturbed expression of genes involved in auxin and ethylene metabolism. The results illustrate the cross talk that can occur between primary and secondary metabolism within transgenic plants.
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