ArticlePlant molecular biology2006
The A and B loci in tobacco regulate a network of stress response genes, few of which are associated with nicotine biosynthesis.
Article in Plant molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 37 citations in OpenAlex.
- Impact of nicotine pathway downregulation on polyamine biosynthesis and leaf ripening in tobacco.Plant direct · 2021Article
- Article
- Polyamines delay leaf maturation in low-alkaloid tobacco varieties.Plant direct · 2018Article
- Identification of Iridoid Glucoside Transporters in Catharanthus roseus.Plant & cell physiology · 2017Article
- Alternative splicing of basic chitinase gene PR3b in the low-nicotine mutants of Nicotiana tabacum L. cv. Burley 21.Journal of experimental botany · 2016Article
- Article
- NtERF32: a non-NIC2 locus AP2/ERF transcription factor required in jasmonate-inducible nicotine biosynthesis in tobacco.Plant molecular biology · 2014Article
- An expanding role for purine uptake permease-like transporters in plant secondary metabolism.Frontiers in plant science · 2012Article
- Tobacco nicotine uptake permease (NUP1) affects alkaloid metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Reactive oxygen species regulate alkaloid metabolism in undifferentiated N. tabacum cells.Plant cell reports · 2010Article
- The A622 gene in Nicotiana glauca (tree tobacco): evidence for a functional role in pyridine alkaloid synthesis.Plant molecular biology · 2009Article
- Multidrug and toxic compound extrusion-type transporters implicated in vacuolar sequestration of nicotine in tobacco roots.Plant physiology · 2009Article
- Generation of tobacco lines with widely different reduction in nicotine levels via RNA silencing approaches.Journal of biosciences · 2008Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotine biosynthesis in Nicotiana tabacum is under genetic control by the A and B loci. Plants containing semi-dominant mutations at both the A and B loci (i.e. aabb genotype) have lower nicotine levels, reduced nicotine biosynthetic enzyme activities, and reduced mRNA levels of the corresponding biosynthetic genes. The A and B loci therefore appear to be coordinate regulators of several nicotine biosynthetic genes and define a group of co-regulated genes called the A-B regulon. To investigate the composition of genes in the A-B regulon, a fluorescent differential display (FDD) screen was used to randomly sample the transcriptomes of wild type and mutant aabb roots. This resulted in the isolation of 64 FDD clones, representing 49 unique genes or gene families. Four genes associated with nicotine biosynthesis were identified, whereas most of the other FDD clones were homologous with an assortment of stress response genes. Thirty-three genes or gene families showed reproducible aabb genotype effects, representing seven distinct mRNA expression patterns in response media treatments that increase the mRNA levels of known alkaloid biosynthetic genes. Thus, the A and B loci regulate the mRNA levels of some target genes differently than others. Eleven genes or gene families showed only treatment-specific effects, representing four mRNA accumulation patterns. These results indicate the A-B regulon is complex network of differentially expressed stress response genes, only a small subset of which are involved in nicotine biosynthesis.
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