ArticleMolecular and cellular biology2006
Functional interaction of CP2 with GATA-1 in the regulation of erythroid promoters.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- A Positive Regulatory Feedback Loop between EKLF/KLF1 and TAL1/SCL Sustaining the Erythropoiesis.International journal of molecular sciences · 2021Article
- The WNT/β-catenin dependent transcription: A tissue-specific business.WIREs mechanisms of disease · 2021Review
- Multiple Integrated Non-clinical Studies Predict the Safety of Lentivirus-Mediated Gene Therapy for β-Thalassemia.Molecular therapy. Methods & clinical development · 2018Article
- Mbd2-CP2c loop drives adult-type globin gene expression and definitive erythropoiesis.Nucleic acids research · 2018Article
- Diagnostic and prognostic relevance of CP2c and YY1 expression in hepatocellular carcinoma.Oncotarget · 2017Article
- GATA1 Activity Governed by Configurations ofFrontiers in oncology · 2016Review
- Screening of Transcription Factors Involved in Fetal Hemoglobin Regulation Using Phylogenetic Footprinting.Evolutionary bioinformatics online · 2015Article
- A regulatory network governing Gata1 and Gata2 gene transcription orchestrates erythroid lineage differentiation.International journal of hematology · 2014Review
- Beta-Actin Is Involved in Modulating Erythropoiesis during Development by Fine-Tuning Gata2 Expression Levels.PloS one · 2013Article
- Contribution of GATA1 dysfunction to multi-step leukemogenesis.Cancer science · 2012Review
- CDC6 expression is regulated by lineage-specific transcription factor GATA1.Cell cycle (Georgetown, Tex.) · 2012Article
- Emx2 is a dose-dependent negative regulator of Sox2 telencephalic enhancers.Nucleic acids research · 2012Article
- Transcriptional regulation by GATA1 and GATA2 during erythropoiesis.International journal of hematology · 2011Article
- Full-length sequence of mouse acupuncture-induced 1-L (aig1l) gene including its transcriptional start site.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- The GATA1-HS2 enhancer allows persistent and position-independent expression of a β-globin transgene.PloS one · 2011Article
- A highly conserved SOX6 double binding site mediates SOX6 gene downregulation in erythroid cells.Nucleic acids research · 2011Article
- PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific alpha-globin expression.Nucleic acids research · 2010Article
- Changes in chromatin structure and methylation of the human interleukin-1beta gene during monopoiesis.Immunology · 2010Article
- Loss of the Gata1 gene IE exon leads to variant transcript expression and the production of a GATA1 protein lacking the N-terminal domain.The Journal of biological chemistry · 2010Article
- Primary sequence and epigenetic determinants of in vivo occupancy of genomic DNA by GATA1.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
We observed that binding sites for the ubiquitously expressed transcription factor CP2 were present in regulatory regions of multiple erythroid genes. In these regions, the CP2 binding site was adjacent to a site for the erythroid factor GATA-1. Using three such regulatory regions (from genes encoding the transcription factors GATA-1, EKLF, and p45 NF-E2), we demonstrated the functional importance of the adjacent CP2/GATA-1 sites. In particular, CP2 binds to the GATA-1 HS2 enhancer, generating a ternary complex with GATA-1 and DNA. Mutations in the CP2 consensus greatly impaired HS2 activity in transient transfection assays with K562 cells. Similar results were obtained by transfection of EKLF and p45 NF-E2 mutant constructs. Chromatin immunoprecipitation with K562 cells showed that CP2 binds in vivo to all three regulatory elements and that both GATA-1 and CP2 were present on the same GATA-1 and EKLF regulatory elements. Adjacent CP2/GATA-1 sites may represent a novel module for erythroid expression of a number of genes. Additionally, coimmunoprecipitation and glutathione S-transferase pull-down experiments demonstrated a physical interaction between GATA-1 and CP2. This may contribute to the functional cooperation between these factors and provide an explanation for the important role of ubiquitous CP2 in the regulation of erythroid genes.
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