ArticleProceedings of the National Academy of Sciences of the United States of America2006
Subcellular localization and functions of the barley stem rust resistance receptor-like serine/threonine-specific protein kinase Rpg1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Engineering plant tandem kinase immune receptors expands effector recognition profiles.Science advances · 2026Article
- Volatiles released by undamaged plants mediate the adaptive growth strategies in neighbors.Journal of experimental botany · 2026Article
- HIPPs of all trades: insights from pathogens on the function of HMA proteins at plasmodesmata.Journal of experimental botany · 2026Review
- A well-annotated genome of Apium graveolens var. dulce cv. Challenger, a celery with resistance to Fusarium oxysporum f. sp. apii race 2.The Plant journal : for cell and molecular biology · 2025Article
- Wheat tandem kinase RWT4 directly binds a fungal effector to activate defense.Nature genetics · 2025Article
- Association mapping unravels the genetic basis for drought related traits in different developmental stages of barley.Scientific reports · 2024Article
- Show me your ID: NLR immune receptors with integrated domains in plants.Essays in biochemistry · 2022Article
- Mutations in a barley cytochrome P450 gene enhances pathogen induced programmed cell death and cutin layer instability.PLoS genetics · 2021Article
- Tandem Protein Kinases Emerge as New Regulators of Plant Immunity.Molecular plant-microbe interactions : MPMI · 2021Review
- Genetic transformation of3 Biotech · 2020Article
- Characterization of genes required for both Rpg1 and rpg4-mediated wheat stem rust resistance in barley.BMC genomics · 2019Article
- The early response during the interaction of fungal phytopathogen and host plant.Open biology · 2017Review
- Insights into the Structure, Function, and Ion-Mediated Signaling Pathways Transduced by Plant Integrin-Linked Kinases.Frontiers in plant science · 2017Article
- Molecular and genetic characterization of barley mutants and genetic mapping of mutant rpr2 required for Rpg1-mediated resistance against stem rust.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Genome-wide association study on stem rust resistance in Kazakh spring barley lines.BMC plant biology · 2016Article
- Fine mapping and identification of a candidate gene for the barley Un8 true loose smut resistance gene.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Concerted action of two avirulent spore effectors activates Reaction to Puccinia graminis 1 (Rpg1)-mediated cereal stem rust resistance.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity.PLoS biology · 2008Article
- Diversification of Lrk/Tak kinase gene clusters is associated with subfunctionalization and cultivar-specific transcript accumulation in barley.Functional & integrative genomics · 2008Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Rpg1 gene confers resistance to many pathotypes of the stem rust fungus Puccinia graminis f. sp. tritici and has protected barley from serious disease losses for over 60 years. Rpg1 encodes a constitutively expressed protein with two tandem kinase domains. Fractionation by differential centrifugation and aqueous two-phase separation of the microsome proteins located Rpg1 mainly in the cytosol but also in the plasma membrane and intracellular membranes. Recombinant Rpg1 autophosphorylates in vitro intramolecularly only serine and threonine amino acids with a preference for Mn(2+) cations and a K(m) of 0.15 and a V(max) of 0.47 nmol.min(-1).mg(-1) protein. The inability of wild-type Rpg1 to transphosphorylate a recombinant Rpg1 inactivated by site-directed mutation confirmed that Rpg1 autophosphorylation proceeds exclusively via an intramolecular mechanism. Site-directed mutagenesis of the two adjacent lysine residues in the ATP anchor of the two-kinase domains established that the first of the two tandem kinase domains is nonfunctional and that lysine 461 of the second domain is the catalytically active residue. Transgenic barley, expressing Rpg1 mutated in either the kinase 1 or 2 domains, were fully susceptible to P. graminis f. sp. tritici revealing requirement of both kinase domains for resistance. In planta-expressed Rpg1 mutant protein confirmed that mutation in domain 2, but not 1, rendered the protein incapable of autophosphorylation.
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