ArticleBiophysical journal2006
Interlaced optical force-fluorescence measurements for single molecule biophysics.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 109 citations in OpenAlex.
- Biophysical and Structural Features of αβT-Cell Receptor Mechanosensing: A Paradigmatic Shift in Understanding T-Cell Activation.Immunological reviews · 2025Review
- Optical traps induce fluorophore photobleaching by two-photon excitation.Biophysical journal · 2023Article
- Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers.International journal of molecular sciences · 2023Review
- Characterizing Biophysical Parameters of Single TCR-pMHC Interactions Using Optical Tweezers.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Optical Force of Bessel Pincer Light-Sheets Beam on a Dielectric Sphere of Arbitrary Size.Nanomaterials (Basel, Switzerland) · 2022Article
- Optical trapping and fluorescence control with vectorial structured light.Scientific reports · 2022Article
- Single Molecule Force Spectroscopy Reveals Distinctions in Key Biophysical Parameters of αβ T-Cell Receptors Compared with Chimeric Antigen Receptors Directed at the Same Ligand.The journal of physical chemistry letters · 2021Article
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- Artificial Cell Membranes Interfaced with Optical Tweezers: A Versatile Microfluidics Platform for Nanomanipulation and Mechanical Characterization.ACS applied materials & interfaces · 2019Article
- High-Performance Image-Based Measurements of Biological Forces and Interactions in a Dual Optical Trap.ACS nano · 2018Article
- Full molecular trajectories of RNA polymerase at single base-pair resolution.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Accounting for polarization in the calibration of a donut beam axial optical tweezers.PloS one · 2018Article
- Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Using the force to find the peptides you're looking for.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mechanosensing drives acuity ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.Methods in molecular biology (Clifton, N.J.) · 2017Article
- High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy.Methods in enzymology · 2017Article
- A programmable DNA origami nanospring that reveals force-induced adjacent binding of myosin VI heads.Nature communications · 2016Article
- Axial Optical Traps: A New Direction for Optical Tweezers.Biophysical journal · 2015Review
- DNA Y structure: a versatile, multidimensional single molecule assay.Nano letters · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Combining optical tweezers with single molecule fluorescence offers a powerful technique to study the biophysical properties of single proteins and molecules. However, such integration into a combined, coincident arrangement has been severely limited by the dramatic reduction in fluorescence longevity of common dyes under simultaneous exposure to trapping and fluorescence excitation beams. We present a novel approach to overcome this problem by alternately modulating the optical trap and excitation beams to prevent simultaneous exposure of the fluorescent dye. We demonstrate the dramatic reduction of trap-induced photobleaching effects on the common single molecule fluorescence dye Cy3, which is highly susceptible to this destructive pathway. The extension in characteristic fluorophore longevity, a 20-fold improvement when compared to simultaneous exposure to both beams, prolongs the fluorescence emission to several tens of seconds in a combined, coincident arrangement. Furthermore, we show that this scheme, interlaced optical force-fluorescence, does not compromise the trap stiffness or single molecule fluorescence sensitivity at sufficiently high modulation frequencies. Such improvement permits the simultaneous measurement of the mechanical state of a system with optical tweezers and the localization of molecular changes with single molecule fluorescence, as demonstrated by mechanically unzipping a 15-basepair DNA segment labeled with Cy3.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.