ArticleFEBS letters2006
Enzymatic activity of the SARS coronavirus main proteinase dimer.
Article in FEBS letters, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Inhibitor-induced dimerization mediates lufotrelvir resistance in mutants of SARS-CoV-2 3C-like protease.Protein science : a publication of the Protein Society · 2025Article
- A naturally occurring G11S mutation in the 3C-like protease from the SARS-CoV-2 virus dramatically weakens the dimer interface.Protein science : a publication of the Protein Society · 2024Article
- Bayesian Inference Elucidates the Catalytic Competency of the SARS-CoV-2 Main Protease 3CLAnalytical chemistry · 2023Article
- Development of a colorimetric assay for the detection of SARS-CoV-2 3CLpro activity.The Biochemical journal · 2022Article
- Unveiling the Effect of Low pH on the SARS-CoV-2 Main Protease by Molecular Dynamics Simulations.Polymers · 2021Article
- Efficiency Improvements and Discovery of New Substrates for a SARS-CoV-2 Main Protease FRET Assay.SLAS discovery : advancing life sciences R & D · 2021Article
- Essentials in saline pharmacology for nasal or respiratory hygiene in times of COVID-19.European journal of clinical pharmacology · 2021Review
- Protein structural heterogeneity: A hypothesis for the basis of proteolytic recognition by the main protease of SARS-CoV and SARS-CoV-2.Biochimie · 2021Article
- Drug Development and Medicinal Chemistry Efforts toward SARS-Coronavirus and Covid-19 Therapeutics.ChemMedChem · 2020Review
- Strategies for Targeting SARS CoV-2: Small Molecule Inhibitors-The Current Status.Frontiers in immunology · 2020Review
- First generation inhibitors of the adenovirus proteinase.FEBS letters · 2013Article
- Activatable Optical Probes for the Detection of Enzymes.Current organic synthesis · 2011Article
- Recent developments in anti-severe acute respiratory syndrome coronavirus chemotherapy.Future virology · 2011Article
- Activation and maturation of SARS-CoV main protease.Protein & cell · 2011Review
- Herpes simplex virus type 1 helicase-primase: DNA binding and consequent protein oligomerization and primase activation.Journal of virology · 2011Article
- Mutation of Asn28 disrupts the dimerization and enzymatic activity of SARS 3CL(pro) .Biochemistry · 2010Article
- Three-dimensional domain swapping as a mechanism to lock the active conformation in a super-active octamer of SARS-CoV main protease.Protein & cell · 2010Article
- Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode.Protein & cell · 2010Article
- Article
- SARS-coronavirus replication/transcription complexes are membrane-protected and need a host factor for activity in vitro.PLoS pathogens · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The enzymatic activity of the SARS coronavirus main proteinase dimer was characterized by a sensitive, quantitative assay. The new, fluorogenic substrate, (Ala-Arg-Leu-Gln-NH)(2)-Rhodamine, contained a severe acute respiratory syndrome coronavirus (SARS CoV) main proteinase consensus cleavage sequence and Rhodamine 110, one of the most detectable compounds known, as the reporter group. The gene for the enzyme was cloned in the absence of purification tags, expressed in Escherichia coli and the enzyme purified. Enzyme activity from the SARS CoV main proteinase dimer could readily be detected at low pM concentrations. The enzyme exhibited a high K(m), and is unusually sensitive to ionic strength and reducing agents.
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