ArticleThe Journal of membrane biology2005
Optical imaging and functional characterization of the transverse tubular system of mammalian muscle fibers using the potentiometric indicator di-8-ANEPPS.
Article in The Journal of membrane biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 49 citations in OpenAlex.
- Simulating the Voltage-Dependent Fluorescence of Di-8-ANEPPS in a Lipid Membrane.The journal of physical chemistry letters · 2023Article
- Optical Recording of Action Potential Initiation and Propagation in Mouse Skeletal Muscle Fibers.Biophysical journal · 2018Article
- Attenuated Ca(2+) release in a mouse model of limb girdle muscular dystrophy 2A.Skeletal muscle · 2016Article
- Alternating bipolar field stimulation identifies muscle fibers with defective excitability but maintained local Ca(2+) signals and contraction.Skeletal muscle · 2016Article
- Na,K-ATPase α2 activity in mammalian skeletal muscle T-tubules is acutely stimulated by extracellular K+.The Journal of general physiology · 2015Article
- Acquiring snapshots of the orientation of trans-membrane protein domains using a hybrid FRET pair.FEBS letters · 2015Article
- Disruption of action potential and calcium signaling properties in malformed myofibers from dystrophin-deficient mice.Physiological reports · 2015Article
- Channelopathies of skeletal muscle excitability.Comprehensive Physiology · 2015Review
- Inward rectifier potassium currents in mammalian skeletal muscle fibres.The Journal of physiology · 2015Article
- Measurement of ATP-Induced Membrane Potential Changes in IVD cells.Cellular and molecular bioengineering · 2014Article
- Action potential-evoked calcium release is impaired in single skeletal muscle fibers from heart failure patients.PloS one · 2014Article
- Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers.American journal of physiology. Cell physiology · 2013Article
- Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS.The Journal of general physiology · 2013Article
- The delayed rectifier potassium conductance in the sarcolemma and the transverse tubular system membranes of mammalian skeletal muscle fibers.The Journal of general physiology · 2012Article
- Voltage clamp methods for the study of membrane currents and SR Ca(2+) release in adult skeletal muscle fibres.Progress in biophysics and molecular biology · 2012Review
- Novel insights into the pathomechanisms of skeletal muscle channelopathies.Current neurology and neuroscience reports · 2012Review
- The Na conductance in the sarcolemma and the transverse tubular system membranes of mammalian skeletal muscle fibers.The Journal of general physiology · 2011Article
- Functional expression of transgenic 1sDHPR channels in adult mammalian skeletal muscle fibres.The Journal of physiology · 2011Article
- Reduced gain of excitation-contraction coupling in triadin-null myotubes is mediated by the disruption of FKBP12/RyR1 interaction.Cell calcium · 2011Article
- Chloride currents from the transverse tubular system in adult mammalian skeletal muscle fibers.The Journal of general physiology · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Potentiometric dyes are useful tools for studying membrane potential changes from compartments inaccessible to direct electrical recordings. In the past, we have combined electrophysiological and optical techniques to investigate, by using absorbance and fluorescence potentiometric dyes, the electrical properties of the transverse tubular system in amphibian skeletal muscle fibers. In this paper we expand on recent observations using the fluorescent potentiometric indicator di-8-ANEPPS to investigate structural and functional properties of the transverse tubular system in mammalian skeletal muscle fibers. Two-photon laser scanning confocal fluorescence images of live muscle fibers suggest that the distance between consecutive rows of transverse tubules flanking the Z-lines remains relatively constant in muscle fibers stretched to attain sarcomere lengths of up to 3.5 microm. Furthermore, the combined use of two-microelectrode electrophysiological techniques with microscopic fluorescence spectroscopy and imaging allowed us to compare the spectral properties of di-8-ANEPPS fluorescence in fibers at rest, with those of fluorescence transients recorded in stimulated fibers. We found that although the indicator has excitation and emission peaks at 470 and 588 nm, respectively, fluorescence transients display optimal fractional changes (13%/100 mV) when using filters to select excitation wavelengths in the 530-550 nm band and emissions beyond 590 nm. Under these conditions, results from tetanically stimulated fibers and from voltage-clamp experiments suggest strongly that, although the kinetics of di-8-ANEPPS transients in mammalian fibers are very rapid and approximate those of the surface membrane electrical recordings, they arise from the transverse tubular system membranes.
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