ArticleThe Journal of membrane biology2005
Properties of new, long-wavelength, voltage-sensitive dyes in the heart.
Article in The Journal of membrane biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 72 citations in OpenAlex.
- Optical mapping of cardiac electromechanics in beating in vivo hearts.Biophysical journal · 2023Article
- Optical Mapping of Cardiomyocytes in Monolayer Derived from Induced Pluripotent Stem Cells.Cells · 2023Review
- Stop the beat to see the rhythm: excitation-contraction uncoupling in cardiac research.American journal of physiology. Heart and circulatory physiology · 2021Review
- Advances and prospects of rhodopsin-based optogenetics in plant research.Plant physiology · 2021Review
- Optical mapping of human embryonic stem cell-derived cardiomyocyte graft electrical activity in injured hearts.Stem cell research & therapy · 2020Article
- What Is the Optimal Light Source for Optical Mapping Using Voltage- and Calcium-Sensitive Dyes?Physiological research · 2020Article
- Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1.iScience · 2020Article
- Cardiac Optogenetics and Optical Mapping - Overcoming Spectral Congestion in All-Optical Cardiac Electrophysiology.Frontiers in physiology · 2019Review
- Examination of the Effects of Conduction Slowing on the Upstroke of Optically Recorded Action Potentials.Frontiers in physiology · 2019Article
- Principles of Optogenetic Methods and Their Application to Cardiac Experimental Systems.Frontiers in physiology · 2019Review
- Optical Mapping of Membrane Potential and Epicardial Deformation in Beating Hearts.Biophysical journal · 2016Article
- A technical review of optical mapping of intracellular calcium within myocardial tissue.American journal of physiology. Heart and circulatory physiology · 2016Review
- Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels.eLife · 2016Article
- The effects of remodeling with heart failure on mode of initiation of ventricular fibrillation and its spatiotemporal organization.Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2015Article
- Mechanism of automaticity in cardiomyocytes derived from human induced pluripotent stem cells.Journal of molecular and cellular cardiology · 2015Article
- Article
- Optical mapping of optogenetically shaped cardiac action potentials.Scientific reports · 2014Article
- A century of optocardiography.IEEE reviews in biomedical engineering · 2014Review
- Optical imaging of voltage and calcium in cardiac cells & tissues.Circulation research · 2012Review
- Optical mapping of action potentials and calcium transients in the mouse heart.Journal of visualized experiments : JoVE · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Membrane potential measurements using voltage-sensitive dyes (VSDs) have made important contributions to our understanding of electrophysiological properties of multi-cellular systems. Here, we report the development of long wavelength VSDs designed to record cardiac action potentials (APs) from deeper layers in the heart. The emission spectrum of styryl VSDs was red-shifted by incorporating a thienyl group in the polymethine bridge to lengthen and retain the rigidity of the chromophore. Seven dyes, Pittsburgh I to IV and VI to VIII (PGH I-VIII) were synthesized and characterized with respect to their spectral properties in organic solvents and heart muscles. PGH VSDs exhibited 2 absorption, 2 excitation and 2 voltage-sensitive emission peaks, with large Stokes shifts (> 100 nm). Hearts (rabbit, guinea pig and Rana pipiens) and neurohypophyses (CD-1 mice) were effectively stained by injecting a bolus (10-50 microl) of stock solution of VSD (2-5 mM) dissolved in in dimethylsulfoxide plus low molecular weight Pluronic (16% of L64). Other preparations were better stained with a bolus of VSD (2-5 mM) Tyrode's solution at pH 6.0. Action spectra measured with a fast CCD camera showed that PGH I exhibited an increase in fractional fluorescence, DeltaF/F = 17.5 % per AP at 720 nm with 550 nm excitation and DeltaF/F = - 6% per AP at 830 nm with 670 nm excitation. In frog hearts, PGH1 was stable with approximately 30% decrease in fluorescence and AP amplitude during 3 h of intermittent excitation or 1 h of continuous high intensity excitation (300 W Xe-Hg Arc lamp), which was attributed to a combination of dye wash out > photobleaching > dynamic damage > run down of the preparation. The long wavelengths, large Stokes shifts, high DeltaF/F and low baseline fluorescence make PGH dyes a valuable tool in optical mapping and for simultaneous mapping of APs and intracellular Ca(2+).
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Registered trials
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