ArticleThe Journal of membrane biology2005
Changes in FAD and NADH fluorescence in neurosecretory terminals are triggered by calcium entry and by ADP production.
Article in The Journal of membrane biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism.Journal of biomedical optics · 2024Article
- Use of thiacalix[4]arene C-1193 for a directed influence on the functional activity of mitochondria and simulation of this process using a Petri nets.Biotechnologia · 2024Article
- Optical Imaging Demonstrates Tissue-Specific Metabolic Perturbations in Mblac1 Knockout Mice.IEEE journal of translational engineering in health and medicine · 2024Article
- Birefringence Changes of Dendrites in Mouse Hippocampal Slices Revealed with Polarizing Microscopy.Biophysical journal · 2020Article
- Glycolysis selectively shapes the presynaptic action potential waveform.Journal of neurophysiology · 2016Article
- Rapid Postnatal Expansion of Neural Networks Occurs in an Environment of Altered Neurovascular and Neurometabolic Coupling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Multiple cytosolic calcium buffers in posterior pituitary nerve terminals.The Journal of general physiology · 2016Article
- An auditory colliculothalamocortical brain slice preparation in mouse.Journal of neurophysiology · 2014Article
- Carbon monoxide inhalation increases microparticles causing vascular and CNS dysfunction.Toxicology and applied pharmacology · 2013Article
- Microparticles generated by decompression stress cause central nervous system injury manifested as neurohypophysial terminal action potential broadening.Journal of applied physiology (Bethesda, Md. : 1985) · 2013Article
- In vivo imaging of brain metabolism activity using a phosphorescent oxygen-sensitive probe.Journal of neuroscience methods · 2013Article
- Cytosolic calcium coordinates mitochondrial energy metabolism with presynaptic activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Use of NAD(P)H and flavoprotein autofluorescence transients to probe neuron and astrocyte responses to synaptic activation.Neurochemistry international · 2010Review
- Spatial Relationship between Flavoprotein Fluorescence and the Hemodynamic Response in the Primary Visual Cortex of Alert Macaque Monkeys.Frontiers in neuroenergetics · 2010Article
- Synthesis of a new NIR fluorescent Nd complex labeling agent.Journal of fluorescence · 2010Article
- Differences in O2 availability resolve the apparent discrepancies in metabolic intrinsic optical signals in vivo and in vitro.Trends in neurosciences · 2007Review
- Mechanical spikes from nerve terminals.Biophysical journal · 2007Article
- Stimulation-induced changes in NADH fluorescence and mitochondrial membrane potential in lizard motor nerve terminals.The Journal of physiology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We measured changes in the intrinsic fluorescence (IF) of the neurosecretory terminals of the mouse neurohypophysis during brief (1-2 s) trains of stimuli. With fluorescence excitation at either 350 +/- 20 or 450 +/- 50 nm, and with emission measured, respectively, at 450 +/- 50 or > or = 520 nm, DeltaF/F(o) was approximately 5-8 % for a 2 s train of 30 action potentials. The IF changes lagged the onset of stimulation by approximately 100 ms and were eliminated by 1 microM tetrodotoxin (TTX). The signals were partially inhibited by 500 microM Cd(2+), by substitution of Mg(2+) for Ca(2+), by Ca(2+)-free Ringer's with 0.5 mM EGTA, and by 50 microM ouabain. The IF signals were also sensitive to the mitochondrial metabolic inhibitors CCCP (0.3 microM), FCCP (0.3 microM), and NaN(3) (0.3 mM), and their amplitude reflected the partial pressure of oxygen (pO(2)) in the bath. Resting fluorescence at both 350 nm and 450 nm exhibited significant bleaching. Flavin adenine dinucleotide (FAD) is fluorescent, while its reduced form FADH(2) is relatively non-fluorescent; conversely, NADH is fluorescent, while its oxidized form NAD is non-fluorescent. Thus, our experiments suggest that the stimulus-coupled rise in [Ca(2+)](i) triggers an increase in FAD and NAD as FADH(2) and NADH are oxidized, but that elevation of [Ca(2+)](i), alone cannot account for the totality of changes in intrinsic fluorescence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.