ArticleThe Journal of molecular diagnostics : JMD2006
Glioma test array for use with formalin-fixed, paraffin-embedded tissue: array comparative genomic hybridization correlates with loss of heterozygosity and fluorescence in situ hybridization.
Article in The Journal of molecular diagnostics : JMD, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Diagnostic accuracy of 1p/19q codeletion tests in oligodendroglioma: A comprehensive meta-analysis based on a Cochrane systematic review.Neuropathology and applied neurobiology · 2022Pooled it
- Diagnostic test accuracy and cost-effectiveness of tests for codeletion of chromosomal arms 1p and 19q in people with glioma.The Cochrane database of systematic reviews · 2022Pooled it
- Genomic aberrations associated with outcome in anaplastic oligodendroglial tumors treated within the EORTC phase III trial 26951.Journal of neuro-oncology · 2011Trial
- EWSR1-PATZ1-rearranged sarcoma: a report of nine cases of spindle and round cell neoplasms with predilection for thoracoabdominal soft tissues and frequent expression of neural and skeletal muscle markers.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2021Article
- Determination of genetic aberrations and novel transcripts involved in the pathogenesis of oligodendroglioma using array comparative genomic hybridization and next generation sequencing.Oncology letters · 2019Article
- Prognostic significance of 1p36 locus deletion in adenoid cystic carcinoma of the salivary glands.Virchows Archiv : an international journal of pathology · 2018Article
- Decoupling genetics, lineages, and microenvironment in IDH-mutant gliomas by single-cell RNA-seq.Science (New York, N.Y.) · 2017Article
- The Utilization of Formalin Fixed-Paraffin-Embedded Specimens in High Throughput Genomic Studies.International journal of genomics · 2017Review
- Article
- HER2 expression identifies dynamic functional states within circulating breast cancer cells.Nature · 2016Article
- Elevation of Urinary 2-Hydroxyglutarate in IDH-Mutant Glioma.The oncologist · 2016Article
- Automated Analysis of 1p/19q Status by FISH in Oligodendroglial Tumors: Rationale and Proposal of an Algorithm.PloS one · 2015Article
- ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.PloS one · 2014Article
- Transparency of reporting in molecular diagnostics.International journal of molecular sciences · 2013Article
- Maintenance of primary tumor phenotype and genotype in glioblastoma stem cells.Neuro-oncology · 2012Article
- Genomic changes in gliomas detected using single nucleotide polymorphism array in formalin-fixed, paraffin-embedded tissue: superior results compared with microsatellite analysis.The Journal of molecular diagnostics : JMD · 2011Article
- Genome-wide comparison of paired fresh frozen and formalin-fixed paraffin-embedded gliomas by custom BAC and oligonucleotide array comparative genomic hybridization: facilitating analysis of archival gliomas.Acta neuropathologica · 2011Article
- Assessing Population Level Genetic Instability via Moving Average.Statistics in biosciences · 2010Article
- A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.Cancer research · 2010Article
- Array-based genomics in glioma research.Brain pathology (Zurich, Switzerland) · 2010Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Array-based comparative genomic hybridization (aCGH) is a powerful, high-throughput tool for whole genome analysis. Until recently, aCGH could only be reproducibly performed on frozen tissue samples and with significant tissue amounts. For brain tumors however, paraffin-embedded tissue blocks from small stereotactic biopsies may be the only tissue routinely available. The development of methods to analyze formalin-fixed, paraffin-embedded (FFPE) material therefore has the potential to impact molecular diagnosis in a significant way. To this end, we constructed a BAC array representing chromosomes 1, 7, 19, and X because 1p/19q deletion and EGFR gene amplification provide clinically relevant information for glioma diagnosis. We also optimized a two-step labeling procedure using an amine-modified nucleotide for generating aCGH probes. Using this approach, we analyzed a series of 28 FFPE oligodendroglial tumors for alterations of chromosomes 1, 7, and 19. We also independently assayed these tumors for 1p/19q deletion by fluorescence in situ hybridization and by loss of heterozygosity analyses. The concordance between aCGH, standard loss of heterozygosity and fluorescence in situ hybridization was nearly 100% for the chromosomes analyzed. These results suggest that aCGH could offer an improved molecular diagnostic approach for gliomas because of its ability to detect clinically relevant molecular alterations in small FFPE specimens.
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