ArticleThe Journal of molecular diagnostics : JMD2006
Diagnosis of human congenital cytomegalovirus infection by amplification of viral DNA from dried blood spots on perinatal cards.
Article in The Journal of molecular diagnostics : JMD, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Dried blood spots PCR assays to screen congenital cytomegalovirus infection: a meta-analysis.Virology journal · 2015Pooled it
- Saliva polymerase-chain-reaction assay for cytomegalovirus screening in newborns.The New England journal of medicine · 2011Trial
- Neonatal screening for congenital cytomegalovirus infection in Tehran, Iran, using Guthrie cards.Iranian journal of microbiology · 2020Article
- Article
- Newborn Dried Blood Spot Polymerase Chain Reaction to Identify Infants with Congenital Cytomegalovirus-Associated Sensorineural Hearing Loss.The Journal of pediatrics · 2017Article
- Innovations in health and demographic surveillance systems to establish the causal impacts of HIV policies.Current opinion in HIV and AIDS · 2015Review
- Genotype distribution, viral load and clinical characteristics of infants with postnatal or congenital cytomegalovirus infection.PloS one · 2014Article
- Quantitation of cytomegalovirus DNA load in dried blood spots correlates well with plasma viral load.Journal of clinical microbiology · 2013Article
- The "silent" global burden of congenital cytomegalovirus.Clinical microbiology reviews · 2013Review
- Article
- Active retinitis in an infant with postnatally acquired cytomegalovirus infection.Journal of perinatology : official journal of the California Perinatal Association · 2012Article
- Overview of the diagnosis of cytomegalovirus infection.Infectious disorders drug targets · 2011Article
- Article
- Comparison of DNA extraction methods from small samples of newborn screening cards suitable for retrospective perinatal viral research.Journal of biomolecular techniques : JBT · 2011Article
- Public health and laboratory considerations regarding newborn screening for congenital cytomegalovirus.Journal of inherited metabolic disease · 2010Review
- Article
- Evaluation of different cytomegalovirus (CMV) DNA PCR protocols for analysis of dried blood spots from consecutive cases of neonates with congenital CMV infections.Journal of clinical microbiology · 2008Article
- Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection.Journal of clinical microbiology · 2007Article
- Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Congenital human cytomegalovirus (HCMV) infection affects 1% of children and is the most common infectious cause of sensorineural hearing loss. Due to the difficulty of diagnosing deafness and other neurological disorders in infants, affected individuals may not be recognized until much later when active infection has resolved and culture is no longer informative. To overcome this problem, congenital HCMV infection was diagnosed retrospectively by testing residual blood samples collected from newborns and dried on perinatal cards as part of the North Carolina Newborn Screening Program. We modified the Qiagen method for purifying DNA from dried blood spots to increase the sample size and recovery of the lysate. A multiplex, real-time TaqMan polymerase chain reaction assay on an ABI 7900 instrument measured a highly conserved segment of the HCMV polymerase gene and the APOB human control gene. HCMV DNA was detected in blood dried on perinatal cards from all seven infants with culture-proven congenital infection, and all 24 negative control cases lacked detectable HCMV DNA. Our findings suggest that it is possible to diagnose congenital HCMV infection using dried blood collected up to 20 months earlier. Further studies are warranted on patients with hearing loss or other neurological deficits to determine the percentage that is attributable to congenital HCMV infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.