ArticleThe Journal of physiology2006
Activation of the cGMP/PKG pathway inhibits electrical activity in rabbit urethral interstitial cells of Cajal by reducing the spatial spread of Ca2+ waves.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Ca²⁺ signaling in myenteric interstitial cells of Cajal (ICC-MY) and their role as conditional pacemakers in the colon.Cell calcium · 2025Article
- Article
- New open-source software for subcellular segmentation and analysis of spatiotemporal fluorescence signals using deep learning.iScience · 2022Article
- A high throughput machine-learning driven analysis of CaCell calcium · 2020Article
- Pacemaker function and neural responsiveness of subserosal interstitial cells of Cajal in the mouse colon.The Journal of physiology · 2020Article
- Tonic inhibition of murine proximal colon is due to nitrergic suppression of CaScientific reports · 2019Article
- Cyclophilin D regulates neuronal activity-induced filopodiagenesis by fine-tuning dendritic mitochondrial calcium dynamics.Journal of neurochemistry · 2018Article
- Spontaneous Ca(2+) transients in interstitial cells of Cajal located within the deep muscular plexus of the murine small intestine.The Journal of physiology · 2016Article
- The role of Ca(2+) influx in spontaneous Ca(2+) wave propagation in interstitial cells of Cajal from the rabbit urethra.The Journal of physiology · 2015Article
- Calcium signalling in Cajal-like interstitial cells of the lower urinary tract.Nature reviews. Urology · 2014Review
- Interstitial cells: regulators of smooth muscle function.Physiological reviews · 2014Review
- The effect of high [K(+)]o on spontaneous Ca(2+) waves in freshly isolated interstitial cells of Cajal from the rabbit urethra.Physiological reports · 2014Article
- Interplay of hydrogen sulfide and nitric oxide on the pacemaker activity of interstitial cells of cajal from mouse small intestine.Chonnam medical journal · 2011Article
- P2X receptor currents in smooth muscle cells contribute to nerve mediated contractions of rabbit urethral smooth muscle.The Journal of urology · 2011Article
- Silencing MaxiK activity in corporal smooth muscle cells initiates compensatory mechanisms to maintain calcium homeostasis.The journal of sexual medicine · 2011Article
- Functions of ICC-like cells in the urinary tract and male genital organs.Journal of cellular and molecular medicine · 2010Review
- Novel excitatory effects of adenosine triphosphate on contractile and pacemaker activity in rabbit urethral smooth muscle.The Journal of urology · 2010Article
- Smooth muscle cell calcium activation mechanisms.The Journal of physiology · 2008Review
- Role of mitochondria in modulation of spontaneous Ca2+ waves in freshly dispersed interstitial cells of Cajal from the rabbit urethra.The Journal of physiology · 2008Article
- Long-range axonal calcium sweep induces axon retraction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In the present study we used a combination of patch clamping and fast confocal Ca2+ imaging to examine the effects of activators of the nitric oxide (NO)/cGMP pathway on pacemaker activity in freshly dispersed ICC from the rabbit urethra, using the amphotericin B perforated patch configuration of the patch-clamp technique. The nitric oxide donor, DEA-NO, the soluble guanylyl cyclase activator YC-1 and the membrane-permeant analogue of cGMP, 8-Br-cGMP inhibited spontaneous transient depolarizations (STDs) and spontaneous transient inward currents (STICs) recorded under current-clamp and voltage-clamp conditions, respectively. Caffeine-evoked Cl- currents were unaltered in the presence of SP-8-Br-PET-cGMPs, suggesting that activation of the cGMP/PKG pathway does not block Cl- channels directly or interfere with Ca2+ release via ryanodine receptors (RyR). However, noradrenaline-evoked Cl- currents were attenuated by SP-8-Br-PET-cGMPs, suggesting that activation of cGMP-dependent protein kinase (PKG) may modulate release of Ca2+ via IP3 receptors (IP3R). When urethral interstitial cells (ICC) were loaded with Fluo4-AM (2 microm), and viewed with a confocal microscope, they fired regular propagating Ca2+ waves, which originated in one or more regions of the cell. Application of DEA-NO or other activators of the cGMP/PKG pathway did not significantly affect the oscillation frequency of these cells, but did significantly reduce their spatial spread. These effects were mimicked by the IP3R blocker, 2-APB (100 microm). These data suggest that NO donors and activators of the cGMP pathway inhibit electrical activity of urethral ICC by reducing the spatial spread of Ca2+ waves, rather than decreasing wave frequency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.