ArticleThe EMBO journal2006
When a helicase is not a helicase: dsDNA tracking by the motor protein EcoR124I.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Short-range translocation by a restriction enzyme motor triggers diffusion along DNA.Nature chemical biology · 2024Article
- The SSU Processome Component Utp25p is a Pseudohelicase.microPublication biology · 2022Article
- The NEOtrap - en route with a new single-molecule technique.iScience · 2021Review
- Structural insights into assembly, operation and inhibition of a type I restriction-modification system.Nature microbiology · 2020Article
- A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I.Journal of molecular modeling · 2018Article
- CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases.Nucleic acids research · 2017Article
- The helical domain of the EcoR124I motor subunit participates in ATPase activity and dsDNA translocation.PeerJ · 2017Article
- Translocation-coupled DNA cleavage by the Type ISP restriction-modification enzymes.Nature chemical biology · 2015Article
- Two-subunit DNA escort mechanism and inactive subunit bypass in an ultra-fast ring ATPase.eLife · 2015Article
- DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis.Nucleic acids research · 2014Article
- Type I restriction enzymes and their relatives.Nucleic acids research · 2014Review
- The helicase-like domains of type III restriction enzymes trigger long-range diffusion along DNA.Science (New York, N.Y.) · 2013Article
- DNA cleavage by Type ISP Restriction-Modification enzymes is initially targeted to the 3'-5' strand.Nucleic acids research · 2013Article
- Dissociation from DNA of Type III Restriction-Modification enzymes during helicase-dependent motion and following endonuclease activity.Nucleic acids research · 2012Article
- Nicking enzyme-based internal labeling of DNA at multiple loci.Nature protocols · 2012Article
- DNA cleavage site selection by Type III restriction enzymes provides evidence for head-on protein collisions following 1D bidirectional motion.Nucleic acids research · 2011Article
- An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme.Nucleic acids research · 2011Article
- Translocation, switching and gating: potential roles for ATP in long-range communication on DNA by Type III restriction endonucleases.Biochemical Society transactions · 2011Review
- Efficient preparation of internally modified single-molecule constructs using nicking enzymes.Nucleic acids research · 2011Article
- Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity.Nucleic acids research · 2011Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Using a combination of single molecule and bulk solution measurements, we have examined the DNA translocation activity of a helicase, the Type I restriction modification enzyme EcoR124I. We find that EcoR124I can translocate past covalent interstrand crosslinks, inconsistent with an obligatory unwinding mechanism. Instead, translocation of the intact dsDNA occurs principally via contacts to the sugar-phosphate backbone and bases of the 3'-5' strand; contacts to the 5'-3' strand are not essential for motion but do play a key role in stabilising the motor on the DNA. A model for dsDNA translocation is presented that could be applicable to a wide range of other enzyme complexes that are also labelled as helicases but which do not have actual unwinding activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.