ArticleGut2006
Global analysis of the human gastric epithelial transcriptome altered by Helicobacter pylori eradication in vivo.
Article in Gut, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Article
- Ubiquitination of ADRα1d/SerpinA1 complex stimulates hypoxia to induce gastric tumorigenesis with a combination of Helicobacter pylori and chronic stress through IL-1α.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2022Article
- Multi-omics reveals microbiome, host gene expression, and immune landscape in gastric carcinogenesis.iScience · 2022Article
- Role of TNF-α-Inducing Protein Secreted byToxins · 2021Review
- Gastrokine 2 Regulates the Antitumor Effect of JAK2/STAT3 Pathway in Gastric Cancer.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- Coordinate expression loss ofAmerican journal of physiology. Gastrointestinal and liver physiology · 2020Article
- Induction and Prevention of Gastric Cancer with CombinedCancers · 2020Article
- GKN2 promotes oxidative stress-induced gastric cancer cell apoptosis via the Hsc70 pathway.Journal of experimental & clinical cancer research : CR · 2019Article
- Article
- Beta-defensins and analogs in Helicobacter pylori infections: mRNA expression levels, DNA methylation, and antibacterial activity.PloS one · 2019Article
- Glycosylation-related gene expression in HT29-MTX-E12 cells upon infection byWorld journal of gastroenterology · 2017Article
- Gastrokine-2 suppresses epithelial mesenchymal transition through PI3K/AKT/GSK3β signaling in gastric cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- A possible role of GDDR in the development of Helicobacter pylori-associated gastric cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Gastrointestinal Factor GDDR Attenuates Epithelial-Mesenchymal Transition in Gastric Cancer via Inhibiting AKT Signal.Digestive diseases and sciences · 2016Article
- Loss of gastrokine-2 drives premalignant gastric inflammation and tumor progression.The Journal of clinical investigation · 2016Article
- A Novel Peptide for Simultaneously Enhanced Treatment of Head and Neck Cancer and Mitigation of Oral Mucositis.PloS one · 2016Article
- Trophoblast expression dynamics of the tumor suppressor gene gastrokine 2.Histochemistry and cell biology · 2015Article
- Effects of gastrokine‑2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways.Molecular medicine reports · 2014Article
- Early or late antibiotic intervention prevents Helicobacter pylori-induced gastric cancer in a mouse model.Cancer letters · 2014Article
- Gastrokine 1 inhibits the carcinogenic potentials of Helicobacter pylori CagA.Carcinogenesis · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
objectiveThe transcriptional profile of gastric epithelial cell lines cocultured with Helicobacter pylori and the global gene expression of whole gastric mucosa has been described previously. We aimed to overcome limitations of previous studies by determining the effects of H pylori eradication on the transcriptome of purified human gastric epithelium using each patient as their own control.
designLaser capture microdissection (LCM) was used to extract mRNA from paraffin-embedded antral epithelium from 10 patients with peptic ulcer disease, before and after H pylori eradication. mRNA was reverse transcribed and applied on to Affymetrix cDNA microarray chips customised for formalin-fixed tissue. Differentially expressed genes were identified and a subset validated by real-time polymerase chain reaction (PCR).
resultsA total of 13 817 transcripts decreased and 9680 increased after H pylori eradication. Applying cut-off criteria (p<0.02, fold-change threshold 2.5) reduced the sample to 98 differentially expressed genes. Genes detected included those previously implicated in H pylori pathophysiology such as interleukin 8, chemokine ligand 3, beta defensin and somatostatin, as well as novel genes such as GDDR (TFIZ1), chemokine receptors 7 and 8, and gastrokine.
conclusionsLCM of archival specimens has enabled the identification of gastric epithelial genes whose expression is considerably altered after H pylori eradication. This study has confirmed the presence of genes previously implicated in the pathogenesis of H pylori, as well as highlighted novel candidates for further investigation.
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