ArticleBMC microbiology2006
Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing.
Article in BMC microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed.
- Human Q fever outbreak investigation and management in six European countries: a One Health compendium of practices and recommendations.CMI communications · 2026Review
- Molecular detection ofVeterinary and animal science · 2026Article
- Molecular and Serological Detection of Coxiella burnetii in Small Ruminants in Northeastern Brazil.Veterinary medicine and science · 2026Article
- Accelerated Genomic Evolution and Divergence ofMicroorganisms · 2026Article
- Impact ofFrontiers in veterinary science · 2026Article
- Combination of serology and PCR analysis of environmental samples to assessApplied and environmental microbiology · 2025Article
- Genotyping of Coxiella burnetii isolates in Iran using Multiple-locus variable-number tandem repeat analysis (MLVA).BMC microbiology · 2025Article
- A Comprehensive Review of the Mechanisms of Human Q Fever: Pathogenesis and Pathophysiology.Pathogens (Basel, Switzerland) · 2025Review
- Q Fever Vaccines: Unveiling the Historical Journey and Contemporary Innovations in Vaccine Development.Vaccines · 2025Review
- Molecular characterisation ofFrontiers in veterinary science · 2025Article
- Using participatory epidemiology to investigate the causes of cattle abortion in Southwest Ethiopia.Heliyon · 2024Article
- Genotyping and phylogenetic analysis of Coxiella burnetii in domestic ruminant and clinical samples in Iran: insights into Q fever epidemiology.Scientific reports · 2023Article
- A Novel Marine MammalPathogens (Basel, Switzerland) · 2023Article
- Detection ofVeterinarni medicina · 2023Article
- Genotyping ofGenes · 2022Article
- Novel genotypes of Coxiella burnetii circulating in rats in Yunnan Province, China.BMC veterinary research · 2022Article
- Serological, Molecular Prevalence and Genotyping ofVeterinary sciences · 2022Article
- Article
- Molecular detection of Coxiella burnetii in small ruminants and genotyping of specimens collected from goats in Poland.BMC veterinary research · 2021Article
- Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype ofVeterinary sciences · 2021Article
9 more citing papers are in PubMed but not listed here.
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCoxiella burnetii, the causative agent of Q fever, has a wide host range. Few epidemiological tools are available, and they are often expensive or not easily standardized across laboratories. In this work, C. burnetii isolates from livestock and ticks were typed using infrequent restriction site-PCR (IRS-PCR) and multiple loci variable number of tandem repeats (VNTR) analysis (MLVA).
resultsBy applying IRS-PCR, 14 C. burnetii isolates could be divided into six groups containing up to five different isolates. Clustering as deduced from MLVA typing with 17 markers provided an increased resolution with an excellent agreement to IRS-PCR, and with the plasmid type of each strain. MLVA was then applied to 28 additional C. burnetii isolates of different origin and 36 different genotypes were identified among the 42 isolates investigated. The clustering obtained is in agreement with published Multiple Locus Sequence Typing (MLST) data. Two panels of markers are proposed, panel 1 which can be confidently typed on agarose gel at a lower cost and in any laboratory setting (10 minisatellite markers with a repeat unit larger than 9 bp), and panel 2 which comprises 7 microsatellites and provides a higher discriminatory power.
conclusionOur analyses demonstrate that MLVA is a powerful and promising molecular typing tool with a high resolution and of low costs. The consistency of the results with independent methods suggests that MLVA can be applied for epidemiological studies. The resulting data can be queried on a dedicated MLVA genotyping Web service.
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