ArticleInsect molecular biology2006
High efficiency site-specific genetic engineering of the mosquito genome.
Article in Insect molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
73 citing papers in PubMed, 155 citations in OpenAlex.
- Mosquito-based malaria control through genetic, microbial and immunological innovations.Nature reviews. Microbiology · 2026Review
- Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants.Nature communications · 2025Article
- History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- The Development and Expansion of in vivo Germline Editing Technologies in Arthropods: Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) and Beyond.Integrative and comparative biology · 2023Review
- Comprehensive characterization of a transgene insertion in a highly repetitive, centromeric region ofPathogens and global health · 2023Article
- Optimizing CRE and PhiC31 mediated recombination inFrontiers in bioengineering and biotechnology · 2023Article
- The impact of transgenesis on mosquito fitness: A review.Frontiers in insect science · 2022Review
- Development of a pan-neuronal genetic driver inCell reports methods · 2021Article
- Heterogeneous expression of the ammonium transporter AgAmt in chemosensory appendages of the malaria vector, Anopheles gambiae.Insect biochemistry and molecular biology · 2020Article
- The Making of TransgenicMethods and protocols · 2020Article
- How to turn an organism into a model organism in 10 'easy' steps.The Journal of experimental biology · 2020Review
- Experimental population modification of the malaria vector mosquito, Anopheles stephensi.PLoS genetics · 2019Article
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- Isolation and identification of Asaia sp. in Anopheles spp. mosquitoes collected from Iranian malaria settings: steps toward applying paratransgenic tools against malaria.Parasites & vectors · 2018Article
- Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?International journal of environmental research and public health · 2017Review
- nanos-Driven expression of piggyBac transposase induces mobilization of a synthetic autonomous transposon in the malaria vector mosquito, Anopheles stephensi.Insect biochemistry and molecular biology · 2017Article
- Cre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti.Scientific reports · 2017Article
- Review
- Insect transformation with piggyBac: getting the number of injections just right.Insect molecular biology · 2016Article
- Evolutionary biology and genetic techniques for insect control.Evolutionary applications · 2016Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Current techniques for the genetic engineering of insect genomes utilize transposable genetic elements, which are inefficient, have limited carrying capacity and give rise to position effects and insertional mutagenesis. As an alternative, we investigated two site-specific integration mechanisms in the yellow fever mosquito, Aedes aegypti. One was a modified CRE/lox system from phage P1 and the other a viral integrase system from Streptomyces phage phi C31. The modified CRE/lox system consistently failed to produce stable germline transformants but the phi C31 system was highly successful, increasing integration efficiency by up to 7.9-fold. The ability to efficiently target transgenes to specific chromosomal locations and the potential to integrate very large transgenes has broad applicability to research on many medically and economically important species.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.