ArticlePLoS biology2006
Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.
Article in PLoS biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 86 citations in OpenAlex.
- Promoter identity shapes splicing outcomes and fidelity.Nature communications · 2026Article
- Emerging and re-emerging themes in co-transcriptional pre-mRNA splicing.Molecular cell · 2024Review
- Pre-mRNA splicing and its cotranscriptional connections.Trends in genetics : TIG · 2023Review
- Studying the connection between SF3B1 and four types of cancer by analyzing networks constructed based on published research.Scientific reports · 2023Article
- Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.International journal of molecular sciences · 2021Review
- mTOR-coordinated Post-Transcriptional Gene Regulations: from Fundamental to Pathogenic Insights.Journal of lipid and atherosclerosis · 2020Review
- Functional impacts of non-coding RNA processing on enhancer activity and target gene expression.Journal of molecular cell biology · 2019Review
- Long-read sequencing of nascent RNA reveals coupling among RNA processing events.Genome research · 2018Article
- Transcriptome analysis of hypoxic cancer cells uncovers intron retention in EIF2B5 as a mechanism to inhibit translation.PLoS biology · 2017Article
- FUS functions in coupling transcription to splicing by mediating an interaction between RNAP II and U1 snRNP.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Splicing of many human genes involves sites embedded within introns.Nucleic acids research · 2015Article
- Introduction to cotranscriptional RNA splicing.Methods in molecular biology (Clifton, N.J.) · 2014Article
- In vitro systems for coupling RNAP II transcription to splicing and polyadenylation.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Kinetic analysis of in vitro pre-mRNA splicing in HeLa nuclear extract.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Analysis of genetic interaction networks shows that alternatively spliced genes are highly versatile.PloS one · 2013Article
- Dynamic reconfiguration of long human genes during one transcription cycle.Molecular and cellular biology · 2012Article
- Small-scale nuclear extracts for functional assays of gene-expression machineries.Journal of visualized experiments : JoVE · 2012Article
- Modelling reveals kinetic advantages of co-transcriptional splicing.PLoS computational biology · 2011Article
- Differential patterns of intronic and exonic DNA regions with respect to RNA polymerase II occupancy, nucleosome density and H3K36me3 marking in fission yeast.Genome biology · 2011Article
- Integrative regulatory mapping indicates that the RNA-binding protein HuR couples pre-mRNA processing and mRNA stability.Molecular cell · 2011Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
RNA processing is carried out in close proximity to the site of transcription, suggesting a regulatory link between transcription and pre-mRNA splicing. Using an in vitro transcription/splicing assay, we demonstrate that an association of RNA polymerase II (Pol II) transcription and pre-mRNA splicing is required for efficient gene expression. Pol II-synthesized RNAs containing functional splice sites are protected from nuclear degradation, presumably because the local concentration of the splicing machinery is sufficiently high to ensure its association over interactions with nucleases. Furthermore, the process of transcription influences alternative splicing of newly synthesized pre-mRNAs. Because other RNA polymerases do not provide similar protection from nucleases, and their RNA products display altered splicing patterns, the link between transcription and RNA processing is RNA Pol II-specific. We propose that the connection between transcription by Pol II and pre-mRNA splicing guarantees an extended half-life and proper processing of nascent pre-mRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.