ArticlePlant physiology2006
The intracellular fate of a recombinant protein is tissue dependent.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 83 citations in OpenAlex.
- Cell Biology Methods to Study Recombinant Proteins in Seeds.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Optimising expression and extraction of recombinant proteins in plants.Frontiers in plant science · 2022Review
- Glyco-Engineering of Plant-Based Expression Systems.Advances in biochemical engineering/biotechnology · 2021Article
- A Hybrid Approach Enabling Large-Scale Glycomic Analysis of Post-Golgi Vesicles Reveals a Transport Route for Polysaccharides.The Plant cell · 2019Article
- High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza.Transgenic research · 2017Article
- Rice endosperm is cost-effective for the production of recombinant griffithsin with potent activity against HIV.Plant biotechnology journal · 2016Article
- Rice endosperm produces an underglycosylated and potent form of the HIV-neutralizing monoclonal antibody 2G12.Plant biotechnology journal · 2016Article
- The dynamic behavior of storage organelles in developing cereal seeds and its impact on the production of recombinant proteins.Frontiers in plant science · 2014Review
- Trafficking of endoplasmic reticulum-retained recombinant proteins is unpredictable in Arabidopsis thaliana.Frontiers in plant science · 2014Review
- In vivo intracellular pH measurements in tobacco and Arabidopsis reveal an unexpected pH gradient in the endomembrane system.The Plant cell · 2013Article
- Plant species and organ influence the structure and subcellular localization of recombinant glycoproteins.Plant molecular biology · 2013Article
- ER stress response induced by the production of human IL-7 in rice endosperm cells.Plant molecular biology · 2013Article
- Article
- Expression pattern of the alpha-kafirin promoter coupled with a signal peptide from Sorghum bicolor L. Moench.Journal of biomedicine & biotechnology · 2012Article
- Ubiquitin fusion expression and tissue-dependent targeting of hG-CSF in transgenic tobacco.BMC biotechnology · 2011Article
- A fluorescent reporter protein containing AtRMR1 domains is targeted to the storage and central vacuoles in Arabidopsis thaliana and tobacco leaf cells.Plant cell reports · 2011Article
- Accumulation of functional recombinant human coagulation factor IX in transgenic soybean seeds.Transgenic research · 2011Article
- The changing fate of a secretory glycoprotein in developing maize endosperm.Plant physiology · 2010Article
- Expression and subcellular targeting of human insulin-like growth factor binding protein-3 in transgenic tobacco plants.Transgenic research · 2009Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant proteins directed to the secretory pathway in plants require a signal peptide for entry into the endoplasmic reticulum. In the absence of further targeting information, such proteins are generally secreted via the default pathway to the apoplast. This has been well documented in protoplasts and leaf tissue, but the trafficking of recombinant proteins in seeds and other storage tissues has rarely been investigated. We used Aspergillus niger phytase as a model glycoprotein to compare the intracellular fate of a recombinant protein in the leaves and seeds of rice (Oryza sativa). Using fluorescence and electron microscopy we showed that the recombinant protein was efficiently secreted from leaf cells as expected. In contrast, within endosperm cells it was retained in endoplasmic reticulum-derived prolamin bodies and protein storage vacuoles. Consistent with our immunolocalization data, the phytase produced in endosperm cells possessed oligomannose and vacuolar-type N-glycans [Man(3)(Xyl)(Fuc)GlcNAc(2)], whereas the phytase produced in leaves contained predominantly secretion-type N-glycans [GlcNAc(2)Man(3)(Xyl)(Fuc)GlcNAc(2)]. The latter could not be detected in preparations of the endosperm-derived phytase. Our results show that the intracellular deposition and modification of a recombinant protein is tissue dependent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.