ArticleBiophysical journal2006
Simultaneous multicolor fluorescence cross-correlation spectroscopy to detect higher order molecular interactions using single wavelength laser excitation.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Four-color fluorescence cross-correlation spectroscopy with one laser and one camera.Biomedical optics express · 2023Article
- Four-color fluorescence cross-correlation spectroscopy with one laser and one camera.bioRxiv : the preprint server for biology · 2023Article
- Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection.eLife · 2021Article
- Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World.Chemical reviews · 2018Review
- Article
- Probing Temperature- and pH-Dependent Binding between Quantum Dots and Bovine Serum Albumin by Fluorescence Correlation Spectroscopy.Nanomaterials (Basel, Switzerland) · 2017Article
- Characterization of Ternary Protein Systems In Vivo with Tricolor Heterospecies Partition Analysis.Biophysical journal · 2016Article
- Pulsed interleaved excitation fluctuation imaging.Biophysical journal · 2013Article
- Vectorized data acquisition and fast triple-correlation integrals for Fluorescence Triple Correlation Spectroscopy.Computer physics communications · 2013Article
- Quantitation of ten 30S ribosomal assembly intermediates using fluorescence triple correlation spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Single-molecule tools elucidate H2A.Z nucleosome composition.Journal of cell science · 2012Article
- An orange fluorescent protein with a large Stokes shift for single-excitation multicolor FCCS and FRET imaging.Journal of the American Chemical Society · 2012Article
- Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopy.Biophysical journal · 2012Article
- Determining antibody stoichiometry using time-integrated fluorescence cumulant analysis.The journal of physical chemistry. B · 2011Article
- Pin-hole array correlation imaging: highly parallel fluorescence correlation spectroscopy.Biophysical journal · 2009Article
- Determination of in vivo dissociation constant, KD, of Cdc42-effector complexes in live mammalian cells using single wavelength fluorescence cross-correlation spectroscopy.The Journal of biological chemistry · 2009Article
- Studies on interaction of CdTe quantum dots with bovine serum albumin using fluorescence correlation spectroscopy.Journal of fluorescence · 2009Article
- Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopy.Biophysical journal · 2007Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Fluorescence cross-correlation spectroscopy is a powerful method for the study of molecular interactions and dynamics in solution and even in living cells. Usually, in the optical setup, either two laser beams have to be superimposed in their respective confocal volumes or two-photon excitation is used for a dual-color detection system. It has been shown recently that fluorescence cross correlation can be achieved with spectrally similar fluorophores using single wavelength excitation fluorescence cross-correlation spectroscopy (SW-FCCS). In this study, we show that SW-FCCS allows the simultaneous excitation of up to three fluorophores in which the cross correlation of their fluctuation signals is detected separately in three detection channels. The experimental and theoretical model to describe triple pairwise cross correlations incorporating cross talk and possible changes in emission characteristics such as quenching upon binding are outlined. The effectiveness of SW-FCCS to detect binding of three interacting partners is experimentally verified with a standard ligand-receptor model, biotin-streptavidin, where differently labeled biotin ligands and their binding to a third-color labeled streptavidin are studied. The cross-correlation amplitudes and their changes with stoichiometric binding are analyzed and the upper limits of dissociation constants are determined. Performed with appropriate negative controls, SW-FCCS can determine interaction patterns between ligands and receptors.
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