ArticleMalaria journal2006
A high-throughput method for quantifying alleles and haplotypes of the malaria vaccine candidate Plasmodium falciparum merozoite surface protein-1 19 kDa.
Article in Malaria journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Mapping sulphadoxine-pyrimethamine-resistant Plasmodium falciparum malaria in infected humans and in parasite populations in Africa.Scientific reports · 2017Pooled it
- Genetic diversity of Plasmodium falciparum and genetic profile in children affected by uncomplicated malaria in Cameroon.Malaria journal · 2020Trial
- Dynamics in multiplicity of Plasmodium falciparum infection among children with asymptomatic malaria in central Ghana.BMC genetics · 2017Trial
- Estimating true antimalarial efficacy by heteroduplex tracking assay in patients with complex Plasmodium falciparum infections.Antimicrobial agents and chemotherapy · 2007Trial
- Review of MrsFreqPhase methods: methods designed to estimate statistically malaria parasite multiplicity of infection, relatedness, frequency and phase.Malaria journal · 2024Review
- Genetic polymorphism ofAccess microbiology · 2022Article
- Large and finite sample properties of a maximum-likelihood estimator for multiplicity of infection.PloS one · 2018Article
- THE REAL McCOIL: A method for the concurrent estimation of the complexity of infection and SNP allele frequency for malaria parasites.PLoS computational biology · 2017Article
- Persistence of Sulfadoxine-Pyrimethamine Resistance Despite Reduction of Drug Pressure in Malawi.The Journal of infectious diseases · 2015Article
- Maximum likelihood estimation of frequencies of known haplotypes from pooled sequence data.Molecular biology and evolution · 2013Article
- Detecting mutations in PfCRT and PfMDR1 genes among Plasmodium falciparum isolates from Saudi Arabia by pyrosequencing.Parasitology research · 2011Article
- Differentiating Plasmodium falciparum alleles by transforming Cartesian X,Y data to polar coordinates.BMC genetics · 2010Article
- The Plasmodium falciparum merozoite surface protein-1 19 KD antibody response in the Peruvian Amazon predominantly targets the non-allele specific, shared sites of this antigen.Malaria journal · 2010Article
- Drug resistance and genetic mapping in Plasmodium falciparum.Current genetics · 2008Review
- A general SNP-based molecular barcode for Plasmodium falciparum identification and tracking.Malaria journal · 2008Article
- Effects of Plasmodium falciparum mixed infections on in vitro antimalarial drug tests and genotyping.The American journal of tropical medicine and hygiene · 2008Article
- A polymerase chain reaction/ligase detection reaction fluorescent microsphere assay to determine Plasmodium falciparum MSP-119 haplotypes.The American journal of tropical medicine and hygiene · 2007Article
- Short report: rare Plasmodium falciparum merozoite surface protein 1 19-kda (msp-1(19)) haplotypes identified in Mali using high-throughput genotyping methods.The American journal of tropical medicine and hygiene · 2007Article
- Plasmodium falciparum: a novel method for analyzing haplotypes in mixed infections.Experimental parasitology · 2007Article
- Dynamics of polymorphism in a malaria vaccine antigen at a vaccine-testing site in Mali.PLoS medicine · 2007Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundMalaria vaccine efficacy may be compromised if the frequency of non-target alleles increases following vaccination with a genetically polymorphic target. Methods are needed to monitor genetic diversity in polymorphic vaccine antigens, but determining which genetic variants of such antigens are present in infected individuals is complicated by the frequent occurrence of mixed infections.
methodsPyrosequencing was used to determine allele frequencies at each of six single nucleotide polymorphisms in the Plasmodium falciparum blood-stage vaccine antigen merozoite surface protein 1 19 kDa (MSP-119) in field samples from a vaccine-testing site in Mali. Mixtures of MSP-119 clones were created to validate a haplotype-estimating algorithm that uses maximum likelihood methods to determine the most probable combination of haplotypes given the allele frequencies for an infection and the haplotypes known to be circulating in the population.
resultsFourteen unique MSP-119 haplotypes were identified among 351 genotyped infections. After adjustment to a standard curve, Pyrosequencing provided accurate and precise estimates of allele frequencies in mixed infections. The haplotype-estimating algorithm provided accurate estimates of haplotypes in mixed infections containing up to three haplotypes. Based on the MSP-119 locus, approximately 90% of the 351 infections contained two or fewer haplotypes.
conclusionPyrosequencing in conjunction with a haplotype-estimating algorithm provides accurate estimates of haplotypes present in infections with up to 3 haplotypes, and can be used to monitor genetic diversity in parasite populations prior to and following introduction of MSP-1-based malaria vaccines.
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