ArticleMolecular biology of the cell2006
On and off membrane dynamics of the endoplasmic reticulum-golgi tethering factor p115 in vivo.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- The Golgi vesicle tether p115 can bind directly to the ER exit site organiser Sec16A.Journal of cell science · 2026Article
- AtypicalScience advances · 2022Article
- The Role of GM130 in Nervous System Diseases.Frontiers in neurology · 2021Review
- Inactivation of NSF ATPase Leads to Cathepsin B Release After Transient Cerebral Ischemia.Translational stroke research · 2018Article
- Membrane detachment is not essential for COG complex function.Molecular biology of the cell · 2018Article
- Rab1 recruits WHAMM during membrane remodeling but limits actin nucleation.Molecular biology of the cell · 2016Article
- Phospholipase C γ1 regulates early secretory trafficking and cell migration via interaction with p115.Molecular biology of the cell · 2015Article
- p115-SNARE interactions: a dynamic cycle of p115 binding monomeric SNARE motifs and releasing assembled bundles.Traffic (Copenhagen, Denmark) · 2015Article
- Protein kinase A activity is necessary for fission and fusion of Golgi to endoplasmic reticulum retrograde tubules.PloS one · 2015Article
- Apoptosis-linked gene-2 (ALG-2)/Sec31 interactions regulate endoplasmic reticulum (ER)-to-Golgi transport: a potential effector pathway for luminal calcium.The Journal of biological chemistry · 2014Article
- Multipronged interaction of the COG complex with intracellular membranes.Cellular logistics · 2014Article
- Article
- Retrograde vesicle transport in the Golgi.Protoplasma · 2012Review
- Alcohol induces Golgi fragmentation in differentiated PC12 cells by deregulating Rab1-dependent ER-to-Golgi transport.Histochemistry and cell biology · 2012Article
- Identification of a functional domain within the p115 tethering factor that is required for Golgi ribbon assembly and membrane trafficking.Journal of cell science · 2012Article
- Discrete, continuous, and stochastic models of protein sorting in the Golgi apparatus.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- Role of vesicle tethering factors in the ER-Golgi membrane traffic.FEBS letters · 2009Review
- ER-to-Golgi transport by COPII vesicles in Arabidopsis involves a ribosome-excluding scaffold that is transferred with the vesicles to the Golgi matrix.Protoplasma · 2008Article
- Interaction of the conserved oligomeric Golgi complex with t-SNARE Syntaxin5a/Sed5 enhances intra-Golgi SNARE complex stability.The Journal of cell biology · 2007Article
- Progressive motor neuronopathy: a critical role of the tubulin chaperone TBCE in axonal tubulin routing from the Golgi apparatus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms regulating membrane recruitment of the p115 tethering factor in vivo are unknown. Here, we describe cycling of p115 between membranes and cytosol and document the effects of Golgi matrix proteins, Rab1, and soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptors (SNAREs) on this process. Rapid membrane/cytosol exchange is shown by swift (t1/2 approximately 20 s) loss of Golgi-localized p115-green fluorescent protein (GFP) after repeated photobleaching of cell periphery and rapid (t1/2 approximately 13 s) fluorescence recovery after photobleaching Golgi-localized p115-GFP. p115 mutant missing the GM130/giantin binding site exhibits analogous fluorescence recovery after photobleaching (FRAP) (t1/2 approximately 13 s), suggesting that GM130 and giantin are not major determinants of p115 membrane dynamics. In contrast, p115-GFP exchanges more rapidly (t1/2 approximately 8 s) in cells expressing the inactive Rab1/N121I mutant, indicating that p115 cycling is influenced by Rab1. p115-GFP dynamics is also influenced by the assembly status of SNAREs. In cells expressing an ATPase-deficient NSF/E329Q mutant that inhibits SNARE complex disassembly, the cycling kinetics of p115-GFP are significantly slower (t1/2 approximately 21 s). In contrast, in cells incubated at reduced temperature (10 degrees C) that inhibits vesicular traffic, the cycling kinetics of p115-GFP are faster (t1/2 approximately 7 s). These data suggest that p115-binding sites on the membrane are provided by unassembled SNAREs. In agreement, biochemical studies show increased p115 recruitment to membranes in the presence of NSF and alpha-SNAP. Our data support a model in which recruitment of tethers is directly regulated by the assembly status of SNAREs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.