ArticleMolecular biology of the cell2006
Phosphoinositide-3 kinase-Rac1-c-Jun NH2-terminal kinase signaling mediates collagen I-induced cell scattering and up-regulation of N-cadherin expression in mouse mammary epithelial cells.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 85 citations in OpenAlex.
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- Context Matters: Response Heterogeneity to Collagen-Targeting Approaches in Desmoplastic Cancers.Cancers · 2022Review
- Continual Deletion of Spinal Microglia Reforms Astrocyte Scar Favoring Axonal Regeneration.Frontiers in pharmacology · 2022Article
- Cell Heterogeneity and Phenotypic Plasticity in Metastasis Formation: The Case of Colon Cancer.Cancers · 2019Review
- Shotgun label-free proteomic analysis for identification of proteins in HaCaT human skin keratinocytes regulated by the administration of collagen from soft-shelled turtle.Journal of biomedical materials research. Part B, Applied biomaterials · 2018Article
- Environmental cues determine the fate of astrocytes after spinal cord injury.Neural regeneration research · 2017Review
- Collagen 1 signaling at the central nervous system injury site and astrogliosis.Neural regeneration research · 2017Article
- Article
- Interaction of reactive astrocytes with type I collagen induces astrocytic scar formation through the integrin-N-cadherin pathway after spinal cord injury.Nature medicine · 2017Article
- Downregulation of UHRF1 increases tumor malignancy by activating the CXCR4/AKT-JNK/IL-6/Snail signaling axis in hepatocellular carcinoma cells.Scientific reports · 2017Article
- Three-dimensional collagen matrix induces a mechanosensitive invasive epithelial phenotype.Scientific reports · 2017Article
- ECM Composition and Rheology Regulate Growth, Motility, and Response to Photodynamic Therapy in 3D Models of Pancreatic Ductal Adenocarcinoma.Molecular cancer research : MCR · 2017Article
- Up-regulation of N-cadherin by Collagen I-activated Discoidin Domain Receptor 1 in Pancreatic Cancer Requires the Adaptor Molecule Shc1.The Journal of biological chemistry · 2016Article
- The tumor microenvironment: An irreplaceable element of tumor budding and epithelial-mesenchymal transition-mediated cancer metastasis.Cell adhesion & migration · 2016Review
- Receptor activator of NF-κB ligand induces cell adhesion and integrin α2 expression via NF-κB in head and neck cancers.Scientific reports · 2016Article
- Inhibitory effect of quercetin on epithelial to mesenchymal transition in SK-MEL-28 human melanoma cells defined by in vitro analysis on 3D collagen gels.OncoTargets and therapy · 2016Article
- Epithelial cell polarity determinant CRB3 in cancer development.International journal of biological sciences · 2015Review
- Signaling mechanisms of the epithelial-mesenchymal transition.Science signaling · 2014Review
- Tenascin-X promotes epithelial-to-mesenchymal transition by activating latent TGF-β.The Journal of cell biology · 2014Article
- uPAR and cathepsin B-mediated compartmentalization of JNK regulates the migration of glioma-initiating cells.Stem cell research · 2014Article
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3 authors at 2 institutions in 1 country.
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Abstract
During epithelial-to-mesenchymal transitions (EMTs), cells must change their interactions with one another and with their extracellular matrix in a synchronized manner. To characterize signaling pathways cells use to coordinate these changes, we used NMuMG mammary epithelial cells. We showed that these cells become fibroblastic and scattered, with increased N-cadherin expression when cultured on collagen I. Rac1 and c-Jun NH2-terminal kinase (JNK) were activated when cells were plated on collagen I, and dominant inhibitory Rac1 (RacN17) or inhibition of JNK signaling prevented collagen I-induced morphological changes and N-cadherin up-regulation. Furthermore, inhibiting phosphoinositide-3 kinase (PI3K) activity prevented Rac1 and JNK activation as well as collagen I-induced N-cadherin up-regulation. These data implicate PI3K-Rac1-JNK signaling in collagen I-induced changes in NMuMG cells. To establish a role for N-cadherin in collagen I-induced cell scattering, we generated N-cadherin overexpressing and knockdown NMuMG cells and showed that knocking down N-cadherin expression prevented collagen I-induced morphological changes. Motility assays showed that cells overexpressing N-cadherin were significantly more motile than mock-transfected cells and that N-cadherin-mediated motility was collagen I dependent. In addition, we showed that cord formation and branching in three-dimensional culture (EMT-dependent events) required N-cadherin expression and PI3K-Rac1-JNK signaling.
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