ArticleProceedings of the National Academy of Sciences of the United States of America1991
Human papillomavirus 16 E7 protein is associated with the nuclear matrix.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 99 citations in OpenAlex.
- HPV16 E7 Nucleotide Variants Found in Cancer-Free Subjects Affect E7 Protein Expression and Transformation.Cancers · 2022Article
- The Not-So-Good, the Bad and the Ugly: HPV E5, E6 and E7 Oncoproteins in the Orchestration of Carcinogenesis.Viruses · 2021Review
- N-Terminal Fatty Acids of NEFVaccines · 2020Article
- Article
- The papillomavirus E7 proteins.Virology · 2013Review
- Detection of human papillomavirus type 18 E7 oncoprotein in cervical smears: a feasibility study.Journal of clinical microbiology · 2012Article
- Article
- Identification of key amino acid residues that determine the ability of high risk HPV16-E7 to dysregulate major histocompatibility complex class I expression.The Journal of biological chemistry · 2011Article
- Article
- Article
- The alphaherpesvirus US3/ORF66 protein kinases direct phosphorylation of the nuclear matrix protein matrin 3.Journal of virology · 2011Article
- The human papillomavirus E7 oncoprotein.Virology · 2009Review
- Kaposi's sarcoma-associated herpesvirus-encoded LANA can interact with the nuclear mitotic apparatus protein to regulate genome maintenance and segregation.Journal of virology · 2008Article
- USP11 stabilizes HPV-16E7 and further modulates the E7 biological activity.The Journal of biological chemistry · 2008Article
- Different Isoforms of HPV-16 E7 Protein are Present in Cytoplasm and Nucleus.The open virology journal · 2008Article
- Article
- HPV31 E7 facilitates replication by activating E2F2 transcription through its interaction with HDACs.The EMBO journal · 2005Article
- The papillomavirus E7 oncoprotein is ubiquitinated by UbcH7 and Cullin 1- and Skp2-containing E3 ligase.Journal of virology · 2004Article
- Signals that dictate nuclear localization of human papillomavirus type 16 oncoprotein E6 in living cells.Journal of virology · 2003Article
- Human papillomavirus type 16 E7 peptide-directed CD8+ T cells from patients with cervical cancer are cross-reactive with the coronavirus NS2 protein.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
The cellular localization of the human papillomavirus (HPV)-16 E7 gene product in the cell lines CaSki and SiHa has been determined by both biochemical and immunocytochemical methods. These measurements show E7 to be localized in the cell nucleus, specifically with the nonchromatin nuclear structure or nuclear matrix. This localization of E7 required an unambiguous fractionation of the nuclear constituents. This was achieved by using a gentle sequential fractionation procedure to prepare the scaffold consisting of the nuclear matrix and intermediate filaments (NM-IF). Chromatin was cleaved with nuclease and the resulting nucleosomes eluted with 0.25 M ammonium sulfate. Immunostaining of cells after this extraction procedure with monoclonal antibodies (mAbs) to E7 revealed a fine grained, punctate nuclear fluorescence in CaSki and SiHa, which was absent in normal cervical keratinocytes and the HPV-negative cell line C33.1. Western blots of cell fractions with these mAbs showed that E7 was localized in the NM-IF fraction in SiHa and CaSki but was not detected in HPV-negative cells. A second protein of slightly higher mobility is identified by these antisera in HPV-16-containing cells. The data suggest that the previous inability to directly visualize E7 by immunocytology is due to the masking of epitopes by cellular components and not to low levels of protein.
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