ArticleProceedings of the National Academy of Sciences of the United States of America1991
Identification of a 170-kDa protein associated with the vacuolar Na+/H+ antiport of Beta vulgaris.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 53 citations in OpenAlex.
- Endosomal Acid-Base Homeostasis in Neurodegenerative Diseases.Reviews of physiology, biochemistry and pharmacology · 2023Article
- Quantitative gene expression analysis of some sodium ion transporters under salinity stress in Aeluropus littoralis.Saudi journal of biological sciences · 2014Article
- HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants.Trends in plant science · 2009Review
- Regulation by salt of vacuolar H+-ATPase and H+-pyrophosphatase activities and Na+/H+ exchange.Plant signaling & behavior · 2009Review
- Functional validation of a novel isoform of Na+/H+ antiporter from Pennisetum glaucum for enhancing salinity tolerance in rice.Journal of biosciences · 2007Article
- Na+/H+ exchange activity in the plasma membrane of Arabidopsis.Plant physiology · 2003Article
- Expression of water channel proteins in Mesembryanthemum crystallinum.Plant physiology · 2000Article
- Myo-inositol-dependent sodium uptake in ice plantPlant physiology · 1999Article
- Reduced Na+ uptake in the NaCl-hypersensitive sos1 mutant of Arabidopsis thaliana.Plant physiology · 1997Article
- Ion Homeostasis in NaCl Stress Environments.Plant physiology · 1995Article
- Tonoplast Na+/H+ Antiport Activity and Its Energization by the Vacuolar H+-ATPase in the Halophytic Plant Mesembryanthemum crystallinum L.Plant physiology · 1995Article
- Plant Defense Response to Fungal Pathogens (Activation of Host-Plasma Membrane H+-ATPase by Elicitor-Induced Enzyme Dephosphorylation).Plant physiology · 1994Article
- Na+/H+ antiporters, molecular devices that couple the Na+ and H+ circulation in cells.Journal of bioenergetics and biomembranes · 1993Review
- The amiloride resistance gene, car1, of Schizosaccharomyces pombe.Molecular & general genetics : MGG · 1993Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of the addition of amiloride to the growth medium was tested on the Na+/H+ antiport activity of tonoplast vesicles isolated from sugar beet (beta vulgaris L.) cell suspensions. Cells grown in the presence of NaCl and amiloride displayed an increased antiport activity. Analysis of the kinetic data showed that while the affinity of the antiport for Na+ ions did not change, the maximal velocity of the Na+/H+ exchange increased markedly. These results suggest the addition of more antiport molecules to the tonoplast and/or an increase in the turnover rate of the Na+/H+ exchange. The increase in activity of the antiport by the presence of amiloride was correlated with the enhanced synthesis of a tonoplast 170-kDa polypeptide. The increased synthesis of this polypeptide was detected not only upon exposure of the cells to amiloride but also when the cells were exposed to high NaCl concentrations. Polyclonal antibodies against the 170-kDa polypeptide almost completely inhibited the antiport activity. These results suggest the association of the 170-kDa polypeptide with the vacuolar Na+/H+ antiport.
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