ArticleInfection and immunity2006
Attenuated bioluminescent Brucella melitensis mutants GR019 (virB4), GR024 (galE), and GR026 (BMEI1090-BMEI1091) confer protection in mice.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Brucella suppress STING expression via miR-24 to enhance infection.PLoS pathogens · 2020Article
- Article
- Brucella Peptide Cross-Reactive Major Histocompatibility Complex Class I Presentation Activates SIINFEKL-Specific T Cell Receptor-Expressing T Cells.Infection and immunity · 2018Article
- Brucella Genetic Variability in Wildlife Marine Mammals Populations Relates to Host Preference and Ocean Distribution.Genome biology and evolution · 2017Article
- Bioluminescence Imaging of Colonization and Clearance Dynamics of Brucella Suis Vaccine Strain S2 in Mice and Guinea Pigs.Molecular imaging and biology · 2016Article
- Decreased in vivo virulence and altered gene expression by a Brucella melitensis light-sensing histidine kinase mutant.Pathogens and disease · 2015Article
- The invA gene of Brucella melitensis is involved in intracellular invasion and is required to establish infection in a mouse model.Virulence · 2014Article
- Main functions and taxonomic distribution of virulence genes in Brucella melitensis 16 M.PloS one · 2014Article
- Studying host-pathogen interaction events in living mice visualized in real time using biophotonic imaging.Methods in molecular biology (Clifton, N.J.) · 2014Article
- A Cre-Lox P recombination approach for the detection of cell fusion in vivo.Journal of visualized experiments : JoVE · 2012Article
- Brucella melitensis cyclic di-GMP phosphodiesterase BpdA controls expression of flagellar genes.Journal of bacteriology · 2011Article
- Noninvasive biophotonic imaging for studies of infectious disease.FEMS microbiology reviews · 2011Review
- The Brucella abortus phosphoglycerate kinase mutant is highly attenuated and induces protection superior to that of vaccine strain 19 in immunocompromised and immunocompetent mice.Infection and immunity · 2010Article
- Survival of the fittest: how Brucella strains adapt to their intracellular niche in the host.Medical microbiology and immunology · 2009Review
- Nondividing but metabolically active gamma-irradiated Brucella melitensis is protective against virulent B. melitensis challenge in mice.Infection and immunity · 2009Article
- Putative quorum-sensing regulator BlxR of Brucella melitensis regulates virulence factors including the type IV secretion system and flagella.Journal of bacteriology · 2008Article
- Construction of p16Slux, a novel vector for improved bioluminescent labeling of gram-negative bacteria.Applied and environmental microbiology · 2007Article
- Enterohemorrhagic Escherichia coli O157:H7 gal mutants are sensitive to bacteriophage P1 and defective in intestinal colonization.Infection and immunity · 2007Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
In vivo bioluminescence imaging is a persuasive approach to investigate a number of issues in microbial pathogenesis. Previously, we have applied bioluminescence imaging to gain greater insight into Brucella melitensis pathogenesis. Endowing Brucella with bioluminescence allowed direct visualization of bacterial dissemination, pattern of tissue localization, and the contribution of Brucella genes to virulence. In this report, we describe the pathogenicity of three attenuated bioluminescent B. melitensis mutants, GR019 (virB4), GR024 (galE), and GR026 (BMEI1090-BMEI1091), and the dynamics of bioluminescent virulent bacterial infection following vaccination with these mutants. The virB4, galE, and BMEI1090-BMEI1091 mutants were attenuated in interferon regulatory factor 1-deficient (IRF-1(-/-)) mice; however, only the GR019 (virB4) mutant was attenuated in cultured macrophages. Therefore, in vivo imaging provides a comprehensive approach to identify virulence genes that are relevant to in vivo pathogenesis. Our results provide greater insights into the role of galE in virulence and also suggest that BMEI1090 and downstream genes constitute a novel set of genes involved in Brucella virulence. Survival of the vaccine strain in the host for a critical period is important for effective Brucella vaccines. The galE mutant induced no changes in liver and spleen but localized chronically in the tail and protected IRF-1(-/-) and wild-type mice from virulent challenge, implying that this mutant may serve as a potential vaccine candidate in future studies and that the direct visualization of Brucella may provide insight into selection of improved vaccine candidates.
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